Typical Testing Data/Standard Curve (for reference only)
Mouse sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3F ELISA Kit | SEMA3F elisa kit
Mouse Semaphorin-3F, SEMA3F ELISA Kit
Gene Names
Sema3f; Sema4; Semak
Reactivity
Mouse
Synonyms
sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3F; Mouse Semaphorin-3F; SEMA3F ELISA Kit; Mouse Semaphorin-3F (SEMA3F) ELISA kit; SEMA-IV; SEMA4; SEMAK; sema IV; 3F; semaphorin 3F; semaphorin III/F; semaphorin IV; SEMA3F elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse SEMA3F. No significant cross-reactivity or interference between mouse SEMA3F and analogues was observed.
Sequence Length
754
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
< 0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA3F elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,549 Da
NCBI Official Full Name
semaphorin-3F isoform 2
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
NCBI Official Symbol
Sema3f
NCBI Official Synonym Symbols
Sema4; Semak
NCBI Protein Information
semaphorin-3F
UniProt Protein Name
Semaphorin-3F
Protein Family
UniProt Gene Name
Sema3f
UniProt Synonym Gene Names
Sema IV
UniProt Entry Name
SEM3F_MOUSE
Uniprot Description
SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Cellular Component: membrane; extracellular region
Molecular Function: chemorepellent activity; receptor activity
Biological Process: axon extension involved in axon guidance; trigeminal nerve structural organization; axon guidance; facial nerve structural organization; branchiomotor neuron axon guidance; multicellular organismal development; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance; neural crest cell migration
Research Articles on SEMA3F
Similar Products
Product Notes
The Mouse SEMA3F sema3f (Catalog #AAA916704) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA916704 ELISA Kit recognizes Mouse SEMA3F. It is sometimes possible for the material contained within the vial of "sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3F, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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