Mouse netrin 1 ELISA Kit | NTN1 elisa kit

Mouse Netrin-1, NTN1 ELISA Kit

Cat. Num. AAA916051

• Gene Names: Ntn1; AI561871; BC019633; Netrin-1
• Reactivity: Mouse
• Synonyms: netrin 1; Mouse Netrin-1; NTN1 ELISA Kit; Mouse Netrin-1 (NTN1) ELISA kit; NTN1L; Netrin-1; mouse; homolog of; NTN1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse NTN1. No significant cross-reactivity or interference between Mouse NTN1 and analogues was observed.
• Sequence Length: 604

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 3.12 pg/ml-200 pg/ml
• Sensitivity: 0.78 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NTN1 elisa kit

Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for NTN1 has been pre-coated onto a microplate. Add NTN1 (Standards or samples) to the well, and then add Biotin-conjugated NTN1. A competitive inhibition reaction is launched between NTN1 (Standards or samples) and Biotin conjugated NTN1 with the NTN1 antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of NTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 112363094
• NCBI GeneID: 18208
• NCBI Accession #: NP_032770.2
• NCBI GenBank Nucleotide #: NM_008744.2
• UniProt Accession #: O09118; Q60832; Q9QY50; B1ARR0
• Molecular Weight: 67,810 Da
• NCBI Official Full Name: netrin-1
• NCBI Official Synonym Full Names: netrin 1
• NCBI Official Symbol: Ntn1
• NCBI Official Synonym Symbols: AI561871; BC019633; Netrin-1
• NCBI Protein Information: netrin-1
• UniProt Protein Name: Netrin-1
• Protein Family: Netrin
• UniProt Gene Name: Ntn1
• UniProt Entry Name: NET1_MOUSE

Uniprot Description

NTN1: Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: proteinaceous extracellular matrix; cytoplasm; extracellular region; basement membrane

Molecular Function: protein binding

Biological Process: axon guidance; cell-cell adhesion; inner ear morphogenesis; establishment of nucleus localization; mammary gland development; axonogenesis; apoptosis; positive regulation of axon extension; positive regulation of cell proliferation; neuron migration; negative regulation of axon extension; regulation of cell migration; anterior/posterior axon guidance