Rabbit Phospho-HSL-S563 Polyclonal Antibody | anti-HSL-S563 antibody

Phospho-HSL-S563 Rabbit pAb

Cat. Num. AAA9143772

• Gene Names: LIPE; HSL; LHS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: Phospho-HSL-S563; Polyclonal Antibody; Phospho-HSL-S563 Rabbit pAb; LIPE; AOMS4; FPLD6; HSL; LHS; anti-HSL-S563 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-HSL-S563 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Customer Validation: WB: Mouse
• Immunogen: A phospho specific peptide corresponding to residues surrounding S853 of human HSL (NP_005348.2, equivalent to S563 of rat HSL
• Cellular Location: Cell membrane, Cytoplasm, Membrane, caveola, cytosol
• Positive Samples: 3T3L1, H9C2
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using Phospho-HSL-S563 antibody at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37℃.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

Related Product Information for anti-HSL-S563 antibody

Background: The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Product Categories/Family for anti-HSL-S563 antibody

Protein phosphorylation; Research; Endocrine & Metabolism; Lipid Metabolism; Cholesterol Metabolism; Research; Endocrine & Metabolism; Lipid Metabolism; Lipases; Research; Endocrine & Metabolism; AMPK Signaling Pathway; Research; Endocrine & Metabolism; I

NCBI and Uniprot Product Information

• NCBI GI #: 21328446
• NCBI GeneID: 3991
• NCBI Accession #: NP_005348.2
• NCBI GenBank Nucleotide #: NM_005357.2
• UniProt Accession #: Q05469; Q3LRT2; Q6NSL7
• Molecular Weight: 116,598 Da
• NCBI Official Full Name: hormone-sensitive lipase
• NCBI Official Synonym Full Names: lipase, hormone-sensitive
• NCBI Official Symbol: LIPE
• NCBI Official Synonym Symbols: HSL; LHS
• NCBI Protein Information: hormone-sensitive lipase; hormone-sensitive lipase testicular isoform
• UniProt Protein Name: Hormone-sensitive lipase
• UniProt Gene Name: LIPE
• UniProt Synonym Gene Names: HSL
• UniProt Entry Name: LIPS_HUMAN

NCBI Description

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]

Uniprot Description

HSL: hormone sensitive lipase is a lipolytic enzyme of the 'GDXG' family. Plays a rate limiting step in triglyceride lipolysis. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.

Protein type: EC 3.1.1.79; Lipase

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: lipid particle; caveola; cytosol

Molecular Function: triacylglycerol lipase activity; hormone-sensitive lipase activity; protein binding; protein kinase binding

Biological Process: cholesterol metabolic process; triacylglycerol catabolic process; diacylglycerol catabolic process; protein amino acid phosphorylation; lipid catabolic process; long-chain fatty acid catabolic process

Disease: Abdominal Obesity-metabolic Syndrome 4