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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SLC7A9 antibody (MBS9132654) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Rabbit SLC7A9 Polyclonal Antibody | anti-SLC7A9 antibody

SLC7A9 Polyclonal Antibody

Gene Names
SLC7A9; BAT1; CSNU3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Synonyms
SLC7A9; Polyclonal Antibody; SLC7A9 Polyclonal Antibody; BAT1; CSNU3; anti-SLC7A9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
487
Applicable Applications for anti-SLC7A9 antibody
Western Blot (WB), Immunofluorescense (IF)
Application Notes
WB: 1:500 - 1:1000
IF: 1:50-1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human SLC7A9 (NP_055085.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using SLC7A9 antibody (MBS9132654) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SLC7A9 antibody (MBS9132654) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of human kidney cancer using SLC7A9antibody (MBS9132654) at dilution of 1:100.Blue: DAPI for nuclear staining)

Immunofluorescence (IF) (Immunofluorescence analysis of human kidney cancer using SLC7A9antibody (MBS9132654) at dilution of 1:100.Blue: DAPI for nuclear staining)

Immunofluorescence (IF)

(Immunofluorescence analysis of human kidney cancer using SLC7A9 antibody (MBS9132654) at dilution of 1:100.Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of human kidney cancer using SLC7A9 antibody (MBS9132654) at dilution of 1:100.Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of rat kidney using SLC7A9 antibody(MBS9132654) at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of rat kidney using SLC7A9 antibody(MBS9132654) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Related Product Information for anti-SLC7A9 antibody
This gene encodes a protein that belongs to a family of light subunits of amino acid transporters. This protein plays a role in the high-affinity and sodium-independent transport of cystine and neutral and dibasic amino acids, and appears to function in the reabsorption of cystine in the kidney tubule. Mutations in this gene cause non-type I cystinuria, a disease that leads to cystine stones in the urinary system due to impaired transport of cystine and dibasic amino acids. Alternate transcript variants, which encode the same protein, have been found for this gene.
Product Categories/Family for anti-SLC7A9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated: 53kDa
Observed:53kDa
NCBI Official Full Name
b(0,+)-type amino acid transporter 1
NCBI Official Synonym Full Names
solute carrier family 7 member 9
NCBI Official Symbol
SLC7A9
NCBI Official Synonym Symbols
BAT1; CSNU3
NCBI Protein Information
B(0,+)-type amino acid transporter 1
UniProt Protein Name
b(0,+)-type amino acid transporter 1
UniProt Gene Name
SLC7A9
UniProt Synonym Gene Names
BAT1; b(0,+)AT1

NCBI Description

This gene encodes a protein that belongs to a family of light subunits of amino acid transporters. This protein plays a role in the high-affinity and sodium-independent transport of cystine and neutral and dibasic amino acids, and appears to function in the reabsorption of cystine in the kidney tubule. Mutations in this gene cause non-type I cystinuria, a disease that leads to cystine stones in the urinary system due to impaired transport of cystine and dibasic amino acids. Alternate transcript variants, which encode the same protein, have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

SLC7A9: Involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system b(0,+)-like activity). Thought to be responsible for the high- affinity reabsorption of cystine in the kidney tubule. Defects in SLC7A9 are a cause of non-type I cystinuria (CSNU). CSNU arises from impaired transport of cystine and dibasic amino acids through the epithelial cells of the renal tubule and gastrointestinal tract. Three types of cystinuria have been described: type I (fully recessive or silent); type II (high excretor); type III (moderate excretor). Defects in SLC7A9 are associated with type II and type III cystinuria. They also might account for some non-classic type I cystinuria cases. Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 19q13.11

Cellular Component: apical plasma membrane; brush border membrane; integral to plasma membrane; plasma membrane

Molecular Function: antiporter activity; L-cystine transmembrane transporter activity; neutral amino acid transmembrane transporter activity; peptide antigen binding; protein binding

Biological Process: amino acid transport; L-cystine transport; leukocyte migration; neutral amino acid transport; protein complex assembly

Disease: Cystinuria

Research Articles on SLC7A9

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Product Notes

The SLC7A9 slc7a9 (Catalog #AAA9132654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC7A9 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SLC7A9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescense (IF). WB: 1:500 - 1:1000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the SLC7A9 slc7a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC7A9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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