Human Solute Carrier Family 7, Member 9 (SLC7A9) ELISA Kit | SLC7A9 elisa kit

Human Solute Carrier Family 7, Member 9 (SLC7A9) ELISA Kit

Cat. Num. AAA2024923

• Gene Names: SLC7A9; BAT1; CSNU3
• Reactivity: Human
• Synonyms: Solute Carrier Family 7; Member 9 (SLC7A9); Human Solute Carrier Family 7; Member 9 (SLC7A9) ELISA Kit; CSNU3; Cationic Amino Acid Transporter; y+ System; b(0; +)-type amino acid transporter 1; Glycoprotein-associated amino acid transporter b0; +AT1; SLC7A9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 7, Member 9 (SLC7A9).; No significant cross-reactivity or interference between Solute Carrier Family 7, Member 9 (SLC7A9) and analogues was observed.
• Sequence Length: 487

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.55ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 7, Member 9 (SLC7A9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 7, Member 9 (SLC7A9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC7A9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 7, Member 9 (SLC7A9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 7, Member 9 (SLC7A9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 7, Member 9 (SLC7A9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 7, Member 9 (SLC7A9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SLC7A9 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 62897855
• NCBI GeneID: 11136
• Molecular Weight: 53,510 Da
• NCBI Official Full Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 variant, partial
• NCBI Official Synonym Full Names: solute carrier family 7 (amino acid transporter light chain, bo,+ system), member 9
• NCBI Official Symbol: SLC7A9
• NCBI Official Synonym Symbols: BAT1; CSNU3
• NCBI Protein Information: B(0,+)-type amino acid transporter 1
• UniProt Protein Name: Solute carrier family 7 (Cationic amino acid transporter, y+ system), member 9 variant
• Protein Family: b(0,+)-type amino acid transporter
• UniProt Entry Name: Q53FY4_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of light subunits of amino acid transporters. This protein plays a role in the high-affinity and sodium-independent transport of cystine and neutral and dibasic amino acids, and appears to function in the reabsorption of cystine in the kidney tubule. Mutations in this gene cause non-type I cystinuria, a disease that leads to cystine stones in the urinary system due to impaired transport of cystine and dibasic amino acids. Alternate transcript variants, which encode the same protein, have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

SLC7A9: Involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system b(0,+)-like activity). Thought to be responsible for the high- affinity reabsorption of cystine in the kidney tubule. Defects in SLC7A9 are a cause of non-type I cystinuria (CSNU). CSNU arises from impaired transport of cystine and dibasic amino acids through the epithelial cells of the renal tubule and gastrointestinal tract. Three types of cystinuria have been described: type I (fully recessive or silent); type II (high excretor); type III (moderate excretor). Defects in SLC7A9 are associated with type II and type III cystinuria. They also might account for some non-classic type I cystinuria cases. Belongs to the amino acid-polyamine-organocation (APC) superfamily.

Protein type: Membrane protein, integral; Transporter, SLC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: brush border membrane; integral to plasma membrane; apical plasma membrane; plasma membrane

Molecular Function: protein binding; peptide antigen binding; neutral amino acid transmembrane transporter activity; L-cystine transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid transport; L-cystine transport; protein complex assembly; ion transport; blood coagulation; transmembrane transport; leukocyte migration

Disease: Cystinuria