Western Blot (WB)
(Western blot analysis of extracts of HT-29 cells, using SPDEF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
NCBI and Uniprot Product Information
Calculated MW: 35kDa/37kDa
NCBI Description
The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
SPDEF: May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter. Belongs to the ETS family.
Protein type: Transcription factor; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; DNA-binding
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding
Biological Process: transcription from RNA polymerase II promoter; positive regulation of cell fate commitment; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell differentiation; negative regulation of cell fate commitment