Mouse B CELLS Monoclonal Antibody | anti-MS4A1 antibody

MOUSE ANTI HUMAN B CELLS:FITC

Cat. Num. AAA211102

• Gene Names: MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• Applications: Flow Cytometry, Functional Assay
• Synonyms: B CELLS; Monoclonal Antibody; MOUSE ANTI HUMAN B CELLS:FITC; anti-MS4A1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: FMC7
• Specificity: This item recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia.The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al. has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.
• Form/Format: FITC; Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Sequence Length: 130

• Applicable Applications for anti-MS4A1 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ; Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3); 0.2% Bovine Serum Albumin ; Preparation
• Immunogen: HRIK cells - Human B-Lymphoblastoid line; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human B-Cells : FITC)

Related Product Information for anti-MS4A1 antibody

MBS211102 recognises a glycoprotein antigen of 105kD expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes, and has been used widely in the study of B cell malignancy. FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukaemia and hairy cell leukaemia. The nature of the FMC7 antigen has remained poorly defined following its first description in 1981. The expression pattern closely corresponds to that seen with CD22, but studies have shown that FMC7 does not recognise the CD22 molecule itself. A recent publication by Serke et al. has shown that FMC7 recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20.

NCBI and Uniprot Product Information

• NCBI GI #: 23110987
• NCBI GeneID: 931
• NCBI Accession #: NP_068769.2
• NCBI GenBank Nucleotide #: NM_021950.3
• UniProt Accession #: P11836; P08984; Q13963; A6NMS4; B4DT24
• Molecular Weight: 14,581 Da
• NCBI Official Full Name: B-lymphocyte antigen CD20
• NCBI Official Synonym Full Names: membrane-spanning 4-domains, subfamily A, member 1
• NCBI Official Symbol: MS4A1
• NCBI Official Synonym Symbols: B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
• NCBI Protein Information: B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16
• UniProt Protein Name: B-lymphocyte antigen CD20
• Protein Family: B-lymphocyte antigen
• UniProt Gene Name: MS4A1
• UniProt Synonym Gene Names: CD20
• UniProt Entry Name: CD20_HUMAN

NCBI Description

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family.

Protein type: Membrane protein, multi-pass; Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: extracellular space; integral to plasma membrane; external side of plasma membrane

Molecular Function: epidermal growth factor receptor binding

Biological Process: B cell proliferation; humoral immune response

Disease: Immunodeficiency, Common Variable, 5