Typical Testing Data/Standard Curve (for reference only)
Mouse ADAM metallopeptidase with thrombospondin type 1 motif, 5 ELISA Kit | ADAMTS5 elisa kit
Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit
Gene Names
Adamts5; ASMP-2; ADAMTS1; ADAM-TS5; ADAMTS11; AI481094; 9530092O11Rik
Reactivity
Mouse
Synonyms
ADAM metallopeptidase with thrombospondin type 1 motif; 5; Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAMTS5 ELISA Kit; Mouse A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAMTS5 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse ADAMTS5. No significant cross-reactivity or interference between mouse ADAMTS5 and analogues was observed.
Sequence Length
930
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 40 ng/ml.
Sensitivity
The minimum detectable dose of mouse ADAMTS5 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ADAMTS5 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,844 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
NCBI Official Synonym Full Names
a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
NCBI Official Symbol
Adamts5
NCBI Official Synonym Symbols
ASMP-2; ADAMTS1; ADAM-TS5; ADAMTS11; AI481094; 9530092O11Rik
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAMTS-5; ADAM-TS 5; implantin; aggrecanase-2
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
UniProt Gene Name
Adamts5
UniProt Synonym Gene Names
ADAM-TS 5; ADAM-TS5; ADAMTS-5
UniProt Entry Name
ATS5_MOUSE
Uniprot Description
ADAMTS5: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Protein type: Secreted, signal peptide; Extracellular matrix; EC 3.4.24.-; Secreted; Motility/polarity/chemotaxis; Protease
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular region
Molecular Function: peptidase activity; metallopeptidase activity; zinc ion binding; hydrolase activity; metal ion binding; metalloendopeptidase activity
Biological Process: defense response to bacterium; proteolysis
Research Articles on ADAMTS5
Similar Products
Product Notes
The Mouse ADAMTS5 adamts5 (Catalog #AAA901189) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA901189 ELISA Kit recognizes Mouse ADAMTS5. It is sometimes possible for the material contained within the vial of "ADAM metallopeptidase with thrombospondin type 1 motif, 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
