Human Fc fragment of IgG, low affinity IIb, receptor (CD32) ELISA Kit | FCGR2B elisa kit

Human Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B ELISA Kit

Cat. Num. AAA932794

• Gene Names: FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2
• Reactivity: Human
• Synonyms: Fc fragment of IgG; low affinity IIb; receptor (CD32); Human Low affinity immunoglobulin gamma Fc region receptor II-b; FCGR2B ELISA Kit; Human Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) ELISA kit; CD32; CD32B; FCG2; FCGR2; IGFR2; low affinity II; receptor for (CD32) ; receptor; Fc; FCGR2B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FCGR2B. No significant cross-reactivity or interference between human FCGR2B and analogues was observed.
• Sequence Length: 290

• Samples: Serum, plasma, tissue homogenates and cell lysates.
• Detection Range: 0.625 ng/ml -40 ng/ml.
• Sensitivity: The minimum detectable dose of human FCGR2B is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Assay Type: Sandwich
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCGR2B elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCGR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCGR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCGR2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCGR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 50511932
• NCBI GeneID: 2213
• NCBI Accession #: NP_001002273.1
• NCBI GenBank Nucleotide #: NM_001002273.2
• UniProt Accession #: P31994; O95649; Q53X85; Q5VXA9; Q8NIA1; A6H8N3
• Molecular Weight: 34,044 Da
• NCBI Official Full Name: low affinity immunoglobulin gamma Fc region receptor II-b isoform 2
• NCBI Official Synonym Full Names: Fc fragment of IgG, low affinity IIb, receptor (CD32)
• NCBI Official Symbol: FCGR2B
• NCBI Official Synonym Symbols: CD32; FCG2; CD32B; FCGR2; IGFR2
• NCBI Protein Information: low affinity immunoglobulin gamma Fc region receptor II-b; CDw32; fcRII-b; Fc gamma RIIb; fc-gamma-RIIb; fc-gamma RII-b; igG Fc receptor II-b; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• UniProt Protein Name: Low affinity immunoglobulin gamma Fc region receptor II-b
• Protein Family: Low affinity immunoglobulin gamma Fc region receptor
• UniProt Gene Name: FCGR2B
• UniProt Synonym Gene Names: CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b
• UniProt Entry Name: FCG2B_HUMAN

NCBI Description

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Cell surface; Oncoprotein; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; IgG binding

Biological Process: regulation of immune response; viral reproduction; immune response; signal transduction

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To