Typical Testing Data/Standard Curve (for reference only)
Human ADAM metallopeptidase with thrombospondin type 1 motif, 5 ELISA Kit | ADAMTS5 elisa kit
Human A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit
Reactivity
Human
Synonyms
ADAM metallopeptidase with thrombospondin type 1 motif; 5; Human A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAMTS5 ELISA Kit; Human A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAMTS5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human ADAMTS5. No significant cross-reactivity or interference between human ADAMTS5 and analogues was observed.
Sequence Length
207
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
15.6 ng/ml-1000 ng/ml
Sensitivity
< 3.9 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ADAMTS5 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
22,576 Da
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif, 5
NCBI Official Symbol
ADAMTS5
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAM-TS 5; aggrecanase-2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 5
UniProt Gene Name
ADAMTS5
UniProt Synonym Gene Names
ADAM-TS 5; ADAM-TS5; ADAMTS-5
UniProt Entry Name
ATS5_BOVIN
Uniprot Description
Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Catalytic activity: Cleaves aggrecan at the 392-Glu-|-Ala-393 site.
Cofactor: Binds 1 zinc ion per subunit
Research Articles on ADAMTS5
Similar Products
Product Notes
The Human ADAMTS5 adamts5 (Catalog #AAA911846) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA911846 ELISA Kit recognizes Human ADAMTS5. It is sometimes possible for the material contained within the vial of "ADAM metallopeptidase with thrombospondin type 1 motif, 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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