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Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HuvEc cells, using Histamine H1 Receptor (Phospho-Ser398) antibody.)

anti-Human, Mouse Histamine H1 Receptor Antibody | anti-HRH1 antibody

Histamine H1 Receptor (Phospho-Ser398) Antibody

Gene Names
HRH1; H1R; H1-R; HH1R; hisH1
Reactivity
Human, Mouse
Applications
Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Histamine H1 Receptor; Histamine H1 Receptor (Phospho-Ser398) Antibody; H1-R; histamine H1 receptor; anti-HRH1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
Histamine H1 Receptor (Phospho-Ser398) antibody detects endogenous levels of Histamine H1 Receptor only when phosphorylated at serine 398.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
487
Applicable Applications for anti-HRH1 antibody
Immunofluorescence (IF), ELISA (EIA)
Application Notes
IF: 1:100-1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Histamine H1 Receptor around the phosphorylation site of serine 398 (IS-H-SP-R-Q).
All Sites
Human: Ser398
Mouse: Ser399
Preparation and Storage
Store at-20 degree C for 1 year.

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using Histamine H1 Receptor (Phospho-Ser398) antibody.)

Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HuvEc cells, using Histamine H1 Receptor (Phospho-Ser398) antibody.)
References
de Backer M.D., Biochem. Biophys. Res. Commun. 197:1601-1608(1993). Fukui K., Biochem. Biophys. Res. Commun. 201:894-901(1994). Sjoeblom T., Science 314:268-274(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histamine H1 receptor
NCBI Official Synonym Full Names
histamine receptor H1
NCBI Official Symbol
HRH1
NCBI Official Synonym Symbols
H1R; H1-R; HH1R; hisH1
NCBI Protein Information
histamine H1 receptor
UniProt Protein Name
Histamine H1 receptor
Protein Family
UniProt Gene Name
HRH1
UniProt Synonym Gene Names
H1R; HH1R

NCBI Description

Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. It has been associated with multiple processes, including memory and learning, circadian rhythm, and thermoregulation. It is also known to contribute to the pathophysiology of allergic diseases such as atopic dermatitis, asthma, anaphylaxis and allergic rhinitis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2015]

Uniprot Description

In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system.

Research Articles on HRH1

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Product Notes

The HRH1 hrh1 (Catalog #AAA856279) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Histamine H1 Receptor (Phospho-Ser398) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Histamine H1 Receptor can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA). IF: 1:100-1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the HRH1 hrh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histamine H1 Receptor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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