Immunohistochemistry (IHC)
(Immunohistochemistry analysis of human spleen tissue stained with Grp94, mAb (9G10) at 10µg/ml.)
Rat Grp94 Monoclonal Antibody | anti-Endoplasmin; Tra1; Hsp90B1; Gp96 antibody
Grp94 monoclonal antibody (9G10)
Gene Names
HSP90B1; hsp108
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein G affinity purified.
Synonyms
Grp94; Monoclonal Antibody; Grp94 monoclonal antibody (9G10); anti-Endoplasmin; Tra1; Hsp90B1; Gp96 antibody
Host
Rat
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9G10
Specificity
Recognizes human, mouse, rat, bovine, dog, chicken, guinea pig, hamster, monkey, pig, rabbit, sheep and Xenopus Grp94. Detects a band of ~98kDa by Western blot.
Purity/Purification
Protein G affinity purified.
Form/Format
Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
Sequence Length
795
Applicable Applications for anti-Endoplasmin; Tra1; Hsp90B1; Gp96 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB), Electron Microscopy
Application Notes
Immunoprecipitation (1:100)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~98kDa by Western blot.
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~98kDa by Western blot.
Immunogen
Native chicken Grp94.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of human spleen tissue stained with Grp94, mAb (9G10) at 10µg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of human spleen tissue stained with Grp94, mAb (9G10) at 10µg/ml.)
Related Product Information for anti-Endoplasmin; Tra1; Hsp90B1; Gp96 antibody
Grp94 (Glucose-regulated protein 94) is an abundant resident endoplasmic reticulum (ER) lumenal stress protein, which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells.
Product Categories/Family for anti-Endoplasmin; Tra1; Hsp90B1; Gp96 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
91,555 Da
NCBI Official Full Name
Endoplasmin
NCBI Official Synonym Full Names
heat shock protein 90kDa beta (Grp94), member 1
NCBI Official Symbol
HSP90B1
NCBI Official Synonym Symbols
hsp108
NCBI Protein Information
endoplasmin
UniProt Protein Name
Endoplasmin
UniProt Gene Name
HSP90B1
UniProt Synonym Gene Names
TRA1; HSP 108; HSP108
UniProt Entry Name
ENPL_CHICK
Uniprot Description
Molecular chaperone that functions in the processing and transport of secreted proteins. Has ATPase activity ().
Similar Products
Product Notes
The Endoplasmin; Tra1; Hsp90B1; Gp96 hsp90b1 (Catalog #AAA565019) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Grp94 monoclonal antibody (9G10) reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Grp94 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB), Electron Microscopy. Immunoprecipitation (1:100) Western Blot (1:1,000, ECL) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~98kDa by Western blot. Researchers should empirically determine the suitability of the Endoplasmin; Tra1; Hsp90B1; Gp96 hsp90b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Grp94, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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