Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with forskolin (40nM, 30mins), using Vinculin (Ab-821) antibody.)
Vinculin Antibody | anti-VCL antibody
Vinculin (Ab-821) Antibody
Gene Names
Vcl; AA571387; AI462105; AW545629; 9430097D22
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Vinculin; Vinculin (Ab-821) Antibody; Metavinculin; VINC; anti-VCL antibody
Reactivity
Human, Mouse, Rat
Specificity
Vinculin (Ab-821) antibody detects endogenous levels of total Vinculin protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1066
Applicable Applications for anti-VCL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Vinculin around the phosphorylation site of tyrosine 821 (S-G-YP-R-I).
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with forskolin (40nM, 30mins), using Vinculin (Ab-821) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with forskolin (40nM, 30mins), using Vinculin (Ab-821) antibody.)
References
Tricia A. Holwell, J. Lipid Res., Aug 1999; 40: 1440. M Galou, J. Cell Biol., May 1996; 133: 853. PV Hornbeck, J. Immunol., Oct 1993; 151: 4013.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116,717 Da
NCBI Official Full Name
vinculin
NCBI Official Synonym Full Names
vinculin
NCBI Official Symbol
Vcl
NCBI Official Synonym Symbols
AA571387; AI462105; AW545629; 9430097D22
NCBI Protein Information
vinculin
UniProt Protein Name
Vinculin
Protein Family
UniProt Gene Name
Vcl
Uniprot Description
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion ().
Research Articles on VCL
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Product Notes
The VCL vcl (Catalog #AAA8509705) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Vinculin (Ab-821) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vinculin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the VCL vcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vinculin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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