Typical Testing Data/Standard Curve (for reference only)
Rat IL-1beta ELISA Kit | IL-1beta elisa kit
IL-1beta (rat) ELISA Kit
Gene Names
IL1B2; IL1B; IL-1beta
Reactivity
Rat
Synonyms
IL-1beta; IL-1beta (rat) ELISA Kit; IL-1beta elisa kit
Reactivity
Rat
Sequence Length
267
Application Notes
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples
Serum & plasma, Cell Culture Supernatants
Detection Method
Absorbance (450 nm)
Features & Benefits
Easy, convenient and time-saving method to assay IL-1 beta.
The minimum detectable concentration of IL-1 beta is less than 80 pg/ml. •This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha.
The minimum detectable concentration of IL-1 beta is less than 80 pg/ml. •This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-1beta elisa kit
Product Information: IL-1beta (rat) ELISA Kit: Colorimetric Assay for Quantitative measurement of rat IL-1 beta in serum, plasma & cell culture supernatants. Detection Range: 80 pg/ml - 50,000 pg/ml. 100 assays.
Background: MyBioSource's rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta. This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 80 pg/ml. Detection Limit: 80 pg/ml - 50000 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-rat
Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Background: MyBioSource's rat IL-1 beta ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-1 beta. This assay employs an antibody specific for IL-1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1alpha, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3alpha, beta-NGF, TIMP-1, TNF-alpha. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. Sensitivity: 80 pg/ml. Detection Limit: 80 pg/ml - 50000 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-rat
Application- Quantitative protein detection, establishing normal range, validation of antibody array results
Product Categories/Family for IL-1beta elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin 1, beta
NCBI Official Symbol
IL1B2
NCBI Official Synonym Symbols
IL1B; IL-1beta
NCBI Protein Information
interleukin 1, beta
Research Articles on IL-1beta
Similar Products
Product Notes
The Rat IL-1beta (Catalog #AAA843359) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA843359 ELISA Kit recognizes Rat IL-1beta. Quantitative protein detection, establishing normal range, validation of antibody array results. Researchers should empirically determine the suitability of the IL-1beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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