Typical Testing Data/Standard Curve (for reference only)
Galactose and Lactose Assay Kit
Galactose and Lactose Colorimetric / Fluorometric Assay Kit
Synonyms
Galactose and Lactose; Galactose and Lactose Colorimetric / Fluorometric Assay Kit; Galactose and Lactose assay kit
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring levels during fermentation and feeding in protein expression processes
Features & Benefits
Simple procedure; takes ~ 1 hour
Fast and convenient
Kit provides all necessary buffers and reagents for a accurate assay of galactose and lactose individually in various biological samples
Fast and convenient
Kit provides all necessary buffers and reagents for a accurate assay of galactose and lactose individually in various biological samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Galactose and Lactose assay kit
Product Information: Galactose/Lactose Assay Kit: Colorimetric & Fluorometric Assay to Measure Galactose and Lactose levels from various Biological Samples such as Serum, Plasma, other Body Fluids, Food, Growth media, etc. Rapid, Simple & Sensitive. 100 Assays
Background: Galactose (C?H??O? FW: 180.16) and lactose (C??H??O?? FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. MyBioSource's Galactose / Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose - Free Galactose.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Background: Galactose (C?H??O? FW: 180.16) and lactose (C??H??O?? FW: 342.3) are naturally occurring sugars. Lactose consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage. MyBioSource's Galactose / Lactose Assay Kit provides a simple, convenient means for direct measurement of galactose and lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). To detect Galactose, galactose oxidase specifically oxidizes free galactose, generating a product that reacts with the Galactose Probe to produce color (O.D 570nm) and fluorescence (Ex/Em 535/587). To detect lactose, lactose is hydrolyzed by lactase to generate free galactose which is then measured. Thus, Lactose level = Total Galactose - Free Galactose.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Product Categories/Family for Galactose and Lactose assay kit
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Product Notes
The Galactose and Lactose (Catalog #AAA841854) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Galactose and Lactose, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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