Testing Data
Mouse anti-General S- Adenosylhomocysteine Monoclonal Antibody | anti-GARC_3658 antibody
S- Adenosylhomocysteine Antibody (Clone # 301-10)
Reactivity
General
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
>95% Purified from mouse ascites fluid by affinity chromatography.
Synonyms
S- Adenosylhomocysteine; Monoclonal Antibody; S- Adenosylhomocysteine Antibody (Clone # 301-10); anti-GARC_3658 antibody
Host
Mouse
Reactivity
General
Clonality
Monoclonal
Isotype
IgG3
Clone Number
301-10
Specificity
Shows the following reactivites with related compounds: S-Adenoslyhomocysteine: 100%, S-Adenoslymethionine: 1.5 %, Adenosine: 1%, Homocysteine: < 1%, Cysteine: 1%, Glutathione: < 1%, L-Cystathionine: < 1%
Purity/Purification
>95% Purified from mouse ascites fluid by affinity chromatography.
Form/Format
Liquid; 20 mM PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide, 50% Glycerol and 10 mg/ml BSA
Appearance: Colorless liquid
Appearance: Colorless liquid
Sequence Length
322
Applicable Applications for anti-GARC_3658 antibody
cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
cELISA: 1:2000
FCM: 1:100
IHC: 1:100
FCM: 1:100
IHC: 1:100
Immunogen
S-Adenosylhomocysteine conjugated to BSA
Positive Control
IHC: kidney carcinoma tissue, FCM: HepG2 and L02 cell lines
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
Store at 4°C; for long storage, store at 20°C. Avoid multiple freeze-thaw cycles.
Testing Data
Testing Data
Flow Cytometry (FC)
(FCM results from normal liver cell line L02 andhepatocyte carcinoma cell line Hep G2 stained with anti-SAH monoclonal antibody from clone 301-10. Average fluorescence signalin Hep G2 cells was reduced compared to that in L02 cells,indicating SAM level is reduced during carcinogenesis.)
Flow Cytometry (FC)
(FCM results from normal liver cell line L02 andhepatocyte carcinoma cell line Hep G2 stained with anti-SAH monoclonal antibody from clone 301-10. Average fluorescence signalin Hep G2 cells was reduced compared to that in L02 cells,indicating SAM level is reduced during carcinogenesis.)
Flow Cytometry
(FCM analysis control. Normal liver cells L02 andcarcinoma cells Hep G2 were stained with the buffer withoutany antibody.)
Flow Cytometry
(FCM analysis control. Normal liver cells L02 andcarcinoma cells Hep G2 were stained with the buffer withoutany antibody.)
Related Product Information for anti-GARC_3658 antibody
Monoclonal Antibody to detect S-Adenosylhomocysteine in samples.
Background: S-Adenosyl-L-homocysteine (SAH) is an amino acid derivative and an intermediate, by-product, or modulator of several metabolic pathways, including the activated methyl cycle and cysteine biosynthesis. It is also a product of S-adenosyl-methionine (SAM)-dependent methylation of biological molecules, including DNA, RNA, and histones and other proteins. SAH is a risk factor for many diseases, including cancer and neurodegenerative diseases.
Background: S-Adenosyl-L-homocysteine (SAH) is an amino acid derivative and an intermediate, by-product, or modulator of several metabolic pathways, including the activated methyl cycle and cysteine biosynthesis. It is also a product of S-adenosyl-methionine (SAM)-dependent methylation of biological molecules, including DNA, RNA, and histones and other proteins. SAH is a risk factor for many diseases, including cancer and neurodegenerative diseases.
Product Categories/Family for anti-GARC_3658 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
S-adenosylhomocysteine
UniProt Protein Name
S-adenosylhomocysteine
UniProt Gene Name
GARC_3658
UniProt Entry Name
K6YR06_9ALTE
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Product Notes
The S- Adenosylhomocysteine garc_3658 (Catalog #AAA840026) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The S- Adenosylhomocysteine Antibody (Clone # 301-10) reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's S- Adenosylhomocysteine can be used in a range of immunoassay formats including, but not limited to, cELISA (EIA), Flow Ctometry (FC/FACS), Immunohistochemistry (IHC). cELISA: 1:2000 FCM: 1:100 IHC: 1:100. Researchers should empirically determine the suitability of the S- Adenosylhomocysteine garc_3658 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S- Adenosylhomocysteine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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