Testing Data
Rabbit anti-Human, Mouse EHMT1 Polyclonal Antibody | anti-EHMT1 antibody
EHMT1 polyclonal antibody
Appearance: Colorless liquid
Shelf Life: 12 months
Testing Data
Testing Data
Background: EHMT1 is a histone methyltransferase which specifically methylates histone H3 on lysine 9. H3K9 methylation represents a marker for epigenetic transcriptional repression. This repression is established by recruiting HP1 proteins to the methylated histone. H3K9 methylation may contribute to silencing of MYC and E2F responsive genes and plays a role in murine embryogenesis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
EHMT1: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non- histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q- syndrome). Common features seen in these patients are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, flat face with hypertelorism, synophrys, anteverted nares, cupid bow or tented upper lip, everted lower lip, prognathism, macroglossia, conotruncal heart defects, and behavioral problems. Belongs to the histone-lysine methyltransferase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Methyltransferase, protein lysine; Methyltransferase; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation
Chromosomal Location of Human Ortholog: 9q34.3
Cellular Component: nucleoplasm; cytoplasm; plasma membrane; chromosome; nucleus
Molecular Function: methyltransferase activity; protein binding; zinc ion binding; p53 binding; histone lysine N-methyltransferase activity (H3-K9 specific); protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: histone methylation; embryonic development; establishment and/or maintenance of chromatin architecture; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; DNA methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; negative regulation of transcription, DNA-dependent; histone H3-K9 methylation
Disease: Kleefstra Syndrome
Research Articles on EHMT1
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Product Notes
The EHMT1 ehmt1 (Catalog #AAA841331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EHMT1 polyclonal antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EHMT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). ELISA: 1:100 - 1:200, Western Blotting: 1:500. Researchers should empirically determine the suitability of the EHMT1 ehmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EHMT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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