Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Rabbit beta Tubulin 8 Polyclonal Antibody | anti-tub8 antibody
beta Tubulin 8 antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
beta Tubulin 8 antibody was purified by antigen-affinity chromatography
Synonyms
beta Tubulin 8; Polyclonal Antibody; beta Tubulin 8 antibody; Polyclonal beta Tubulin 8; Anti-beta Tubulin 8; FLJ40100; beta Tubulin -8; class VIII beta-tubulin; TUBB8; tubulin beta-8 chain; HSA10p15 beta-tubulin 4Q; anti-tub8 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
beta Tubulin 8 antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
447
Applicable Applications for anti-tub8 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:1000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
ICC:1:100-1:1000
IHC-P: 1:100-1:1000
WB: 1:500-1:3000
Biological Significance
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:H1299) using a 10 % SDS PAGE gel and beta Tubulin 8 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug whole cell lysate (A:A431; B:H1299) using a 10 % SDS PAGE gel and beta Tubulin 8 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded PC14 xenograft using beta Tubulin 8 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded PC14 xenograft using beta Tubulin 8 antibody at a dilution of 1:500)
Related Product Information for anti-tub8 antibody
Affinity purified Rabbit polyclonal beta Tubulin 8 antibody
Product Categories/Family for anti-tub8 antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
50 kDa
NCBI Official Full Name
beta tubulin 8
UniProt Protein Name
Beta tubulin 8
Protein Family
UniProt Gene Name
tub8
UniProt Entry Name
A5CFZ1_HORVD
Uniprot Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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Product Notes
The beta Tubulin 8 tub8 (Catalog #AAA837742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's beta Tubulin 8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:1000 ICC:1:100-1:1000 IHC-P: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the beta Tubulin 8 tub8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta Tubulin 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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