Typical Testing Data/Standard Curve (for reference only)
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Cat Thymidine Kinase 1, Soluble ELISA Kit | TK1 elisa kit
Cat Thymidine Kinase 1, Soluble ELISA
Gene Names
TK1; TK2
Reactivity
Cat
Synonyms
Thymidine Kinase 1; Soluble; Cat Thymidine Kinase 1; Soluble ELISA; TK1 elisa kit
Reactivity
Cat
Sequence Length
234
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.05-20ng/ml
Sensitivity
0.023ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for TK1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Cat Thymidine Kinase 1,Soluble (also known as TK1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Cat TK1 antibody. TK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Cat TK1 Antibody is added and binds to TK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Cat TK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Cat TK1 antibody. TK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Cat TK1 Antibody is added and binds to TK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Cat TK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Thymidine kinase 1, soluble
NCBI Official Synonym Full Names
thymidine kinase 1
NCBI Official Symbol
TK1
NCBI Official Synonym Symbols
TK2
NCBI Protein Information
thymidine kinase, cytosolic
Protein Family
NCBI Description
The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
Research Articles on TK1
Similar Products
Product Notes
The Cat TK1 (Catalog #AAA1603379) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603379 ELISA Kit recognizes Cat TK1. It is sometimes possible for the material contained within the vial of "Thymidine Kinase 1, Soluble, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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