Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Tara antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.)
Rabbit Tara Polyclonal Antibody | anti-Tara antibody
Tara antibody
ICC:1:100-1:1000
WB: 1:500-1:3000
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Tara antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.)
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Tara antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa) using a 10 % SDS PAGE gel and Tara antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa) using a 10 % SDS PAGE gel and Tara antibody at a dilution of 1:1000)
NCBI and Uniprot Product Information
Uniprot Description
Tara: May regulate actin cytoskeletal organization, cell spreading and cell contraction by directly binding and stabilizing filamentous F-actin. The localized formation of TARA and TRIO complexes coordinates the amount of F-actin present in stress fibers. May also serve as a linker protein to recruit proteins required for F-actin formation and turnover. Defects in TRIOBP are the cause of deafness autosomal recessive type 28 (DFNB28). 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: focal adhesion; cytoplasm; microtubule organizing center; midbody; nucleus; actin cytoskeleton
Molecular Function: actin filament binding; ubiquitin protein ligase binding; GTP-Rho binding; myosin II binding
Biological Process: mitosis; actin modification; cell division; barbed-end actin filament capping
Disease: Deafness, Autosomal Recessive 28
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Product Notes
The Tara triobp (Catalog #AAA835183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tara can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF:1:100-1:1000 ICC:1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the Tara triobp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tara, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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