Western Blot (WB)
()
Mouse ICAD Monoclonal Antibody | anti-ICAD antibody
ICAD antibody
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
ICAD antibody was purified by ammonium sulfate precipitation
Synonyms
ICAD; Monoclonal Antibody; ICAD antibody; Monoclonal ICAD; Anti-ICAD; DFFA; DNA fragmentation factor; 45kDa; alpha polypeptide; BOS_15806; DFF1; DFF35; DFF45; DFF-45; DNA fragmentation factor 45 kDa subunit; alpha subunit; DNA fragmentation factor subunit alpha; H13; ICAD-L; ICAD-S; inhibitor of CAD; RP23-121D17.3; anti-ICAD antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AF24G5
Purity/Purification
ICAD antibody was purified by ammonium sulfate precipitation
Form/Format
Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol
Sequence Length
101
Applicable Applications for anti-ICAD antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:2000
IP: 3 ul
IP: 3 ul
Biological Significance
The inhibitor of caspase-3-activated DNase (ICAD) is a caspase 3 substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45 kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35 kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
ICAD antibody was raised in Mouse using a recombinant human protein purified from E Coli (His-ICAD) as the immunogen
Preparation and Storage
Aliquot and store for 1 year at -20 degree C, avoid freeze/thaw cycles
Western Blot (WB)
()
Western Blot (WB)
()
Related Product Information for anti-ICAD antibody
Mouse monoclonal ICAD antibody
Product Categories/Family for anti-ICAD antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
11,787 Da
NCBI Official Full Name
IcaD
UniProt Protein Name
IcaD
Protein Family
UniProt Gene Name
icaD
UniProt Entry Name
G4Y1I5_STACP
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Product Notes
The ICAD icad (Catalog #AAA834449) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ICAD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). WB: 1:2000 IP: 3 ul. Researchers should empirically determine the suitability of the ICAD icad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICAD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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