Rabbit anti-Mouse ICAD Polyclonal Antibody | anti-Dffa antibody

ICAD Antibody

Cat. Num. AAA150237

• Gene Names: Dffa; ICAD; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: ICAD Antibody is affinity chromatography purified via peptide column.
• Synonyms: ICAD; Polyclonal Antibody; ICAD Antibody; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik; Icad; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; DNA fragmentation factor; alpha subunit; anti-Dffa antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ICAD Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 331

• Applicable Applications for anti-Dffa antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected.
• Conjugate: Unconjugated
• Immunogen: ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD.
• Buffer: ICAD Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ICAD antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Testing Data

(Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of ICAD in mouse kidney tissue with ICAD antibody at 5 μg/ml.Green: ICAD antibody (2003)Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-Dffa antibody

ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.

NCBI and Uniprot Product Information

• NCBI GI #: 9087146
• NCBI GeneID: 13347
• UniProt Accession #: O54786; O54787; Q8BQC7; B2KFX0
• Molecular Weight: 45 kDa
• NCBI Official Full Name: DNA fragmentation factor subunit alpha
• NCBI Official Synonym Full Names: DNA fragmentation factor, alpha subunit
• NCBI Official Symbol: Dffa
• NCBI Official Synonym Symbols: ICAD; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik
• NCBI Protein Information: DNA fragmentation factor subunit alpha; inhibitor of CAD; DNA fragmentation factor 45 kDa subunit
• UniProt Protein Name: DNA fragmentation factor subunit alpha
• Protein Family: DNA fragmentation factor
• UniProt Gene Name: Dffa
• UniProt Synonym Gene Names: Icad; DFF-45; ICAD
• UniProt Entry Name: DFFA_MOUSE

Uniprot Description

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; intracellular; cytosol; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis