Western Blot (WB)
(Western Blot analysis of PMA and ionomycin-stimulated (Lane 1) and unstimulated mouse thymocytes (Lane 2) with Nur77 antibody (5 ug/ml) and visualized using Rat anti-Mouse IgG HRP antibody.)
Mouse Nur77 Monoclonal Antibody | anti-Nur77 antibody
Nur77 antibody
Applications
Western Blot
Purity
Nur77 antibody was purified by affinity chromatography.
Synonyms
Nur77; Monoclonal Antibody; Nur77 antibody; Monoclonal Nur77; Anti-Nur77; Nur-77; NGFI-B; Nur 77; NAK1; Nuclear hormone receptor NUR/77; NR4A1; TR3; anti-Nur77 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
12.14
Specificity
Mouse
Purity/Purification
Nur77 antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-Nur77 antibody
Western Blot (WB)
Application Notes
WB: <= 2 ug/ml
It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Biological Significance
This 12.14 monoclonal antibody reacts with mouse Nur77, an inducible orphan nuclear receptor. Expressed in thymocytes and T cell lines, Nur77 promotes apoptosis and plays a role in thymocyte negative selection. Additionally, Nur77 has been shown to be critical for steroid biosynthesis in Leydig cells as well as for the effects of dopamine. In addition, Nur77 has been shown to interact with FoxP3 in regulatory T cells. However, our results with this antibody do not correlate with this observation.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Western Blot (WB)
(Western Blot analysis of PMA and ionomycin-stimulated (Lane 1) and unstimulated mouse thymocytes (Lane 2) with Nur77 antibody (5 ug/ml) and visualized using Rat anti-Mouse IgG HRP antibody.)
Western Blot (WB)
(Western Blot analysis of PMA and ionomycin-stimulated (Lane 1) and unstimulated mouse thymocytes (Lane 2) with Nur77 antibody (5 ug/ml) and visualized using Rat anti-Mouse IgG HRP antibody.)
Related Product Information for anti-Nur77 antibody
Mouse monoclonal Nur77 antibody
Product Categories/Family for anti-Nur77 antibody
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Product Notes
The Nur77 (Catalog #AAA832773) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nur77 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: <= 2 ug/ml It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the Nur77 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nur77, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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