Western Blot (WB)
(IL17 producing cells were generated by culturing human PBMC with Human CD3 antibody and Human CD28 antibody in the presence of Human IL23 protein for 72 hrs, followed by 72 hrs with IL23 only. Day 6 cultures were treated with Brefeldin A (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were analyzed using IL17F antibody (2 ug/ml) and visualized using Mouse IgG HRP antibody.)
Mouse IL17F Monoclonal Antibody | anti-IL17F antibody
IL17F antibody
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest
Western Blot (WB)
(IL17 producing cells were generated by culturing human PBMC with Human CD3 antibody and Human CD28 antibody in the presence of Human IL23 protein for 72 hrs, followed by 72 hrs with IL23 only. Day 6 cultures were treated with Brefeldin A (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were analyzed using IL17F antibody (2 ug/ml) and visualized using Mouse IgG HRP antibody.)
Western Blot (WB)
(IL17 producing cells were generated by culturing human PBMC with Human CD3 antibody and Human CD28 antibody in the presence of Human IL23 protein for 72 hrs, followed by 72 hrs with IL23 only. Day 6 cultures were treated with Brefeldin A (1) or PMA and Ionomycin in the presence of Brefeldin A (2) for 16 hours. Cell lysates were analyzed using IL17F antibody (2 ug/ml) and visualized using Mouse IgG HRP antibody.)
NCBI and Uniprot Product Information
Uniprot Description
IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.
Protein type: Secreted, signal peptide; Secreted; Cytokine
Cellular Component: extracellular space
Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity
Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of interleukin-8 biosynthetic process; regulation of transforming growth factor beta receptor signaling pathway; cartilage development; cytokine biosynthetic process; regulation of interleukin-2 biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; lymphotoxin A biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response
Research Articles on IL17F
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Product Notes
The IL17F il17f (Catalog #AAA838336) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IL17F can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Assay. FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the IL17F il17f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL17F, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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