Mouse KEAP1 Monoclonal Antibody | anti-KEAP1 antibody

KEAP1 antibody

Cat. Num. AAA832739

• Gene Names: KEAP1; INrf2; KLHL19
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: KEAP1 antibody was purified by affinity chromatography.
• Synonyms: KEAP1; Monoclonal Antibody; KEAP1 antibody; Monoclonal KEAP1; Anti-KEAP1; Kelch like ECH associated protein 1; KIAA0132; INrf2; KLHL19; Cytosolic inhibitor of Nrf2; KEAP 1; KEAP-1; anti-KEAP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1G2
• Purity/Purification: KEAP1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 624

• Applicable Applications for anti-KEAP1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC: 1:100; IF: 1:100; WB: 1:2000
• Biological Significance: KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.
• Cross-Reactivity: Human
• Immunogen: KEAP1 antibody was raised in mouse using a full length recombinant protein of human KEAP1 (NP_987096) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant KEAP1 protein (Right) or empty vector (Left) detected with KEAP1 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant KEAP1 protein using KEAP1 antibody)

Related Product Information for anti-KEAP1 antibody

Mouse monoclonal KEAP1 antibody

Product Categories/Family for anti-KEAP1 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 45269145
• NCBI GeneID: 9817
• NCBI Accession #: NP_987096
• NCBI GenBank Nucleotide #: NM_203500.1
• UniProt Accession #: Q14145; Q6LEP0; Q8WTX1; Q9BPY9; B3KPD5
• Molecular Weight: 69,666 Da
• NCBI Official Full Name: kelch-like ECH-associated protein 1
• NCBI Official Synonym Full Names: kelch-like ECH-associated protein 1
• NCBI Official Symbol: KEAP1
• NCBI Official Synonym Symbols: INrf2; KLHL19
• NCBI Protein Information: kelch-like ECH-associated protein 1
• UniProt Protein Name: Kelch-like ECH-associated protein 1
• Protein Family: Kelch-like ECH-associated protein
• UniProt Gene Name: KEAP1
• UniProt Synonym Gene Names: INRF2; KIAA0132; KLHL19; INrf2
• UniProt Entry Name: KEAP1_HUMAN

NCBI Description

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.

Protein type: Transcription regulation; Motility/polarity/chemotaxis; Ubiquitin conjugating system; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; endoplasmic reticulum; cytoplasm; microtubule organizing center; midbody; cytosol

Molecular Function: protein binding; transcription factor binding

Biological Process: transcription, DNA-dependent; in utero embryonic development; negative regulation of transcription factor activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of epidermal cell differentiation