Flow Cytometry (FC/FACS)
(Staining of 3-day unstimulated (left) and 3-day ConA stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control (open histogram) or 0.25 ug of GL7 antibody (filled histogram) followed by Anti-Rat IgM (PE). Total viable cells were used for analysis.)
Rat T and B cell activation Monoclonal Antibody
T and B cell activation antigen antibody
Applications
Western Blot
Purity
T and B cell activation antigen antibody was purified by affinity chromatography.
Synonyms
T and B cell activation; Monoclonal Antibody; T and B cell activation antigen antibody; Monoclonal T and B cell activation antigen; Anti-T and B cell activation antigen; T and B cell activation antigen; Ly-77; GL-7; Ly77; Anti T and B cell activation antigen; anti-T and B cell activation antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
GL-7
Specificity
Mouse
Purity/Purification
T and B cell activation antigen antibody was purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-T and B cell activation antibody
Western Blot (WB)
Application Notes
This hNanog.2 antibody has been tested by immunoblotting of NTERA cell lysates
Biological Significance
This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of 3-day unstimulated (left) and 3-day ConA stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control (open histogram) or 0.25 ug of GL7 antibody (filled histogram) followed by Anti-Rat IgM (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of 3-day unstimulated (left) and 3-day ConA stimulated (right) BALB/c splenocytes with 0.25 ug of Rat IgM kappa Isotype Control (open histogram) or 0.25 ug of GL7 antibody (filled histogram) followed by Anti-Rat IgM (PE). Total viable cells were used for analysis.)
Related Product Information for anti-T and B cell activation antibody
Rat monoclonal T and B cell activation antigen antibody
Product Categories/Family for anti-T and B cell activation antibody
Similar Products
Product Notes
The T and B cell activation (Catalog #AAA832710) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's T and B cell activation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). This hNanog.2 antibody has been tested by immunoblotting of NTERA cell lysates. Researchers should empirically determine the suitability of the T and B cell activation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "T and B cell activation, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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