Immunohistochemistry (IHC)
(FRAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FRAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human FRAT2 Polyclonal Antibody | anti-FRAT2 antibody
FRAT2, CT (FRAT2, GSK-3-binding protein FRAT2, Frequently rearranged in advanced T-cell lymphomas 2) (PE)
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
FRAT2; Polyclonal Antibody; CT (FRAT2; GSK-3-binding protein FRAT2; Frequently rearranged in advanced T-cell lymphomas 2) (PE); Frequently rearranged in advanced T-cell lymphomas 2; anti-FRAT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-FRAT2 antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
FRAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-233 amino acids from the C-terminal region of human FRAT2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(FRAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FRAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(FRAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human bladder carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FRAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Western Blot (WB)
(FRAT2 Antibody (C-term) western blot analysis in ZR-75-1,K562 cell line lysates (35ug/lane).This demonstrates the FRAT2 antibody detected the FRAT2 protein (arrow).)
Western Blot (WB)
(FRAT2 Antibody (C-term) western blot analysis in ZR-75-1,K562 cell line lysates (35ug/lane).This demonstrates the FRAT2 antibody detected the FRAT2 protein (arrow).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of FRAT2 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of FRAT2 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-FRAT2 antibody
The protein encoded by this intronless gene belongs to the GSK-3-binding protein family. Studies show that this protein plays a role as a positive regulator of the WNT signaling pathway. It may be upregulated in tumor progression.
Product Categories/Family for anti-FRAT2 antibody
References
Lee, D.Y., et al. Menopause 17(5):1064-1070(2010). Liu, G.P., et al. Neurobiol. Aging 29(9):1348-1358(2008). Stoothoff, W.H., et al. J. Biol. Chem. 280(1):270-276(2005). Deloukas, P., et al. Nature 429(6990):375-381(2004). Freemantle, S.J., et al. Gene 291 (1-2), 17-27 (2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,051 Da
NCBI Official Full Name
GSK-3-binding protein FRAT2
NCBI Official Synonym Full Names
frequently rearranged in advanced T-cell lymphomas 2
NCBI Official Symbol
FRAT2
NCBI Protein Information
GSK-3-binding protein FRAT2
UniProt Protein Name
GSK-3-binding protein FRAT2
Protein Family
UniProt Gene Name
FRAT2
UniProt Synonym Gene Names
FRAT-2
UniProt Entry Name
FRAT2_HUMAN
NCBI Description
The protein encoded by this intronless gene belongs to the GSK-3-binding protein family. Studies show that this protein plays a role as a positive regulator of the WNT signaling pathway. It may be upregulated in tumor progression. [provided by RefSeq, Jul 2008]
Uniprot Description
FRAT2: Positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3. Belongs to the GSK-3-binding protein family.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 10q24.1
Cellular Component: cytosol
Biological Process: cell proliferation; Wnt receptor signaling pathway; multicellular organismal development
Research Articles on FRAT2
Similar Products
Product Notes
The FRAT2 frat2 (Catalog #AAA6300091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FRAT2, CT (FRAT2, GSK-3-binding protein FRAT2, Frequently rearranged in advanced T-cell lymphomas 2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FRAT2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the FRAT2 frat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FRAT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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