Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.5 ug of Rat IgG1 kappa Isotype Control (FITC) (left) or 0.5 ug of CD150 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
Rat CD150 Monoclonal Antibody | anti-CD150 antibody
CD150 antibody (FITC)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD150; Monoclonal Antibody; CD150 antibody (FITC); Monoclonal CD150; Anti-CD150; SLAM; IPO3; CD 150; CD-150; SLAMF1; IPO-3; anti-CD150 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9D1
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
333
Applicable Applications for anti-CD150 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule. CD150 is expressed by T and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.Mouse hematopoietic stem cells can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150.
Tag/Conjugate
FITC
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.5 ug of Rat IgG1 kappa Isotype Control (FITC) (left) or 0.5 ug of CD150 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.5 ug of Rat IgG1 kappa Isotype Control (FITC) (left) or 0.5 ug of CD150 antibody (FITC) (right). Cells in the lymphocyte gate were used for analysis.)
Related Product Information for anti-CD150 antibody
Rat monoclonal CD150 antibody (FITC)
Product Categories/Family for anti-CD150 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
36,630 Da
NCBI Official Full Name
CD150, partial
UniProt Protein Name
CD150
UniProt Entry Name
K4LB56_CHICK
Similar Products
Product Notes
The CD150 (Catalog #AAA832488) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD150 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD150 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD150, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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