Western Blot (WB)
(Western blot analysis of EVI1 expression in A549 (A), SGC7901 (B) whole cell lysates.)
Rabbit anti-Human EVI1 Polyclonal Antibody | anti-EVI1 antibody
Anti-EVI1 Antibody
Gene Names
MECOM; EVI1; MDS1; PRDM3; MDS1-EVI1; AML1-EVI-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
EVI1; Polyclonal Antibody; Anti-EVI1 Antibody; MDS1; MDS1 and EVI1 complex locus protein MDS1; Myelodysplasia syndrome 1 protein; Myelodysplasia syndrome-associated protein 1; anti-EVI1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of EVI1 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-EVI1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EVI1. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of EVI1 expression in A549 (A), SGC7901 (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of EVI1 expression in A549 (A), SGC7901 (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EVI1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of EVI1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-EVI1 antibody
Rabbit polyclonal antibody to EVI1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
1051
NCBI Official Full Name
MDS1 and EVI1 complex locus protein EVI1 isoform a
NCBI Official Synonym Full Names
MDS1 and EVI1 complex locus
NCBI Official Symbol
MECOM
NCBI Official Synonym Symbols
EVI1; MDS1; PRDM3; MDS1-EVI1; AML1-EVI-1
NCBI Protein Information
MDS1 and EVI1 complex locus protein EVI1; oncogene EVI1; zinc finger protein Evi1; AML1-EVI-1 fusion protein; MDS1 and EVI1 complex locus protein MDS1; myelodysplasia syndrome-associated protein 1; ecotropic virus integration site 1 protein homolog
UniProt Protein Name
MDS1 and EVI1 complex locus protein EVI1
UniProt Gene Name
MECOM
UniProt Synonym Gene Names
EVI1; EVI-1
UniProt Entry Name
EVI1_HUMAN
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Product Notes
The EVI1 mecom (Catalog #AAA8305735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EVI1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EVI1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the EVI1 mecom for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EVI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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