Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ITGa3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Integrin Alpha 3 Polyclonal Antibody | anti-ITGa3 antibody
Integrin Alpha 3 (ITGa3) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ITGa3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ITGa3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Uterus Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Uterus Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a subunit of integrin family of cell surface proteins. The encoded protein undergoes post-translational processing to form a disulfide bond-linked dimer comprised of heavy and light chains. At the cell surface, the encoded protein non-covalently associates with the integrin beta-1 subunit to form a heterodimer that interacts with many extracellular matrix proteins including fibronectin and laminin. Mice lacking the encoded protein die during the first day after birth due to severe abnormalities in kidneys. Mice lacking the encoded protein specifically in the basal layer of epidermis display several skin defects and accelerated wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
Uniprot Description
ITGA3: an integral membrane protein that heterodimerizes with a beta chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. Undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1. Two alternatively spliced isoforms have been described.
Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Chromosomal Location of Human Ortholog: 11 D|11 59.01 cM
Cellular Component: basolateral plasma membrane; cell junction; cell periphery; cell projection; cell surface; excitatory synapse; external side of plasma membrane; extracellular exosome; filopodium membrane; focal adhesion; growth cone; integral component of membrane; integrin alpha3-beta1 complex; integrin complex; invadopodium membrane; membrane; perinuclear region of cytoplasm; plasma membrane; receptor complex; synapse
Molecular Function: collagen binding; fibronectin binding; integrin binding; laminin binding; protease binding; protein binding; protein domain specific binding; protein heterodimerization activity
Biological Process: behavior; cell adhesion; fusion of sperm to egg plasma membrane involved in single fertilization; integrin-mediated signaling pathway; lung development; memory; negative regulation of cell projection organization; negative regulation of Rho protein signal transduction; nephron development; neuron migration; positive regulation of cell-substrate adhesion; positive regulation of epithelial cell migration; positive regulation of gene expression; positive regulation of neuron projection development; regulation of BMP signaling pathway; regulation of transforming growth factor beta receptor signaling pathway; regulation of Wnt receptor signaling pathway; skin development
Research Articles on ITGa3
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Product Notes
The ITGa3 itga3 (Catalog #AAA2032418) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Integrin Alpha 3 (ITGa3) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin Alpha 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ITGa3 itga3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin Alpha 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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