POU4F3 sirna

POU4F3 siRNA (Human)

Cat. Num. AAA828427

• Gene Names: POU4F3; BRN3C; DFNA15
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POU4F3; POU4F3 siRNA (Human); BRN3C; POU domain. class 4. transcription factor 3; Brain-specific homeobox/POU domain protein 3C; Brain-3C; Brn-3C; POU4F3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: POU4F3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 338

• Applicable Applications for POU4F3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human POU4F3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POU4F3 sirna

siRNA to inhibit POU4F3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4505965
• NCBI GeneID: 5459
• NCBI Accession #: NP_002691.1
• NCBI GenBank Nucleotide #: NM_002700.2
• UniProt Accession #: Q15319; O60557; Q2M3F8
• Molecular Weight: 37,052 Da
• NCBI Official Full Name: POU domain, class 4, transcription factor 3
• NCBI Official Synonym Full Names: POU class 4 homeobox 3
• NCBI Official Symbol: POU4F3
• NCBI Official Synonym Symbols: BRN3C; DFNA15
• NCBI Protein Information: POU domain, class 4, transcription factor 3
• UniProt Protein Name: POU domain, class 4, transcription factor 3
• Protein Family: POU domain, class 4, transcription factor
• UniProt Gene Name: POU4F3
• UniProt Synonym Gene Names: BRN3C; Brain-3C; Brn-3C
• UniProt Entry Name: PO4F3_HUMAN

NCBI Description

This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]

Uniprot Description

POU4F3: May play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in POU4F3 are the cause of deafness autosomal dominant type 15 (DFNA15). DFNA15 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the POU transcription factor family. Class- 4 subfamily.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleoplasm

Molecular Function: transcription factor activity

Biological Process: vestibulocochlear nerve development; neuron apoptosis; inner ear morphogenesis; sensory perception of sound; visual perception; axon extension; positive regulation of transcription from RNA polymerase II promoter; auditory receptor cell differentiation; neuromuscular process controlling balance; retinal ganglion cell axon guidance

Disease: Deafness, Autosomal Dominant 15