Human Amiloride-sensitive Cation channel 1, neuronal, ACCN1 ELISA Kit | ACCN1 elisa kit

Human Amiloride-sensitive Cation channel 1, neuronal, ACCN1 ELISA Kit

Cat. Num. AAA2602669

• Gene Names: ASIC2; ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1
• Reactivity: Human
• Synonyms: Amiloride-sensitive Cation channel 1; neuronal; ACCN1; Human Amiloride-sensitive Cation channel 1; ACCN1 ELISA Kit; ACCN1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant ACCN1 concentration
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACCN1 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ACCN1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 9998944
• NCBI GeneID: 40
• NCBI Accession #: NP_001085.2
• NCBI GenBank Nucleotide #: NM_001094.4
• UniProt Accession #: Q16515; Q13553; Q6DJU1; Q8N3E2; E9PBX2
• Molecular Weight: 57,709 Da
• NCBI Official Full Name: acid-sensing ion channel 2 isoform MDEG1
• NCBI Official Synonym Full Names: acid-sensing (proton-gated) ion channel 2
• NCBI Official Symbol: ASIC2
• NCBI Official Synonym Symbols: ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1
• NCBI Protein Information: acid-sensing ion channel 2; brain sodium channel 1; mammalian degenerin homolog; neuronal amiloride-sensitive cation channel 1; amiloride-sensitive cation channel 1, neuronal
• UniProt Protein Name: Acid-sensing ion channel 2
• UniProt Gene Name: ASIC2
• UniProt Synonym Gene Names: ACCN; ACCN1; BNAC1; MDEG; ASIC2; BNC1; BNaC1
• UniProt Entry Name: ASIC2_HUMAN

NCBI Description

This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Feb 2012]

Uniprot Description

ASIC2: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate. Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ASIC2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Apoptosis; Transporter, ion channel; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell soma; integral to plasma membrane; dendritic spine; plasma membrane; synapse

Molecular Function: amiloride-sensitive sodium channel activity; protein binding; ion gated channel activity; voltage-gated sodium channel activity

Biological Process: central nervous system development; regulation of systemic arterial blood pressure by aortic arch baroreceptor feedback; monovalent inorganic cation transport; sensory perception of sour taste; detection of mechanical stimulus involved in sensory perception; peripheral nervous system development; synaptic transmission; positive regulation of synaptogenesis; regulation of membrane potential; sensory perception of sound; regulation of gene expression; regulation of vasoconstriction; regulation of blood coagulation; phototransduction; transmembrane transport; negative regulation of apoptosis