CASP1 sirna

CASP1 siRNA (Mouse)

Cat. Num. AAA827021

• Gene Names: Casp1; ICE; Il1bc
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CASP1; CASP1 siRNA (Mouse); IL1BC; Caspase-1; CASP-1; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45; CASP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CASP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 402

• Applicable Applications for CASP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CASP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CASP1 sirna

siRNA to inhibit CASP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 86198305
• NCBI GeneID: 12362
• NCBI Accession #: NP_033937.2
• NCBI GenBank Nucleotide #: NM_009807.2
• UniProt Accession #: P29452
• Molecular Weight: 45,640 Da
• NCBI Official Full Name: caspase-1
• NCBI Official Synonym Full Names: caspase 1
• NCBI Official Symbol: Casp1
• NCBI Official Synonym Symbols: ICE; Il1bc
• NCBI Protein Information: caspase-1
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: Casp1
• UniProt Synonym Gene Names: Il1bc; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_MOUSE

Uniprot Description

CASP1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18. Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. Specifically inhibited by the cowpox virus Crma protein. Belongs to the peptidase C14A family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.36; Apoptosis; Protease

Cellular Component: neuron projection; protein complex; mitochondrion; cytoplasm; extracellular region; nucleus

Molecular Function: peptidase activity; protein binding; hydrolase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; positive regulation of apoptosis; membrane hyperpolarization; microglial cell activation; interleukin-1 beta production; response to lipopolysaccharide; mitochondrial depolarization; positive regulation of interleukin-1 beta secretion; proteolysis; response to organic cyclic substance; memory; response to ATP; regulation of apoptosis; positive regulation of interleukin-1 alpha secretion; response to bacterium; regulation of inflammatory response; response to hypoxia; protein processing; regulation of autophagy; positive regulation of circadian sleep/wake cycle, non-REM sleep; myoblast fusion; positive regulation of cytokine secretion