MAN1B1 recombinant protein

Recombinant Human MAN1B1 Protein

Cat. Num. AAA8249425

• Gene Names: MAN1B1; MRT15; ERMAN1; ERManI; MANA-ER
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: MAN1B1; Recombinant Human MAN1B1 Protein; Endoplasmic reticulum mannosyl-oligosaccharide 1; 2-alpha-mannosidase; ER alpha-1; 2-mannosidase; ER mannosidase 1; ERMan1; Man9GlcNAc2-specific-processing alpha-mannosidase; Mannosidase alpha class 1B member 1; MAN1B1 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 50mM Tris-HCI, 10mM reduced Glutathione, pH 8.0.
• Sequence: DHWKALAFRL EEEQKMRPEI AGLKPANPPV LPAPQKADTD PENLPEISSQ KTQRHIQRGP PHLQIRPPSQ DLKDGTQEEA TKRQEAPVDP RPEGDPQRTV ISWRGAVIEP EQGTELPSRR AEVPTKPPLP PARTQGTPVH LNYRQKGVID VFLHAWKGYR KFAWGHDELK PVSRSFSEWF GLGLTLIDAL DTMWILGLRK EFEEARKWVS KKLHFEKDVD VNLFESTIRI LGGLLSAYHL SGDSLFLRKA EDFGNRLMPA FRTPSKIPYS DVNIGTGVAH PPRWTSDSTV AEVTSIQLEF RELSRLTGDK KFQEAVEKVT QHIHGLSGKK DGLVPMFINT HSGLFTHLGV FTLGARADSY YEYLLKQWIQ GGKQETQLLE DYVEAIEGVR THLLRHSEPS KLTFVGELAH GRFSAKMDHL VCFLPGTLAL GVYHGLPASH MELAQELMET CYQMNRQMET GLSPEIVHFN LYPQPGRRDV EVKPADRHNL LRPETVESLF YLYRVTGDRK YQDWGWEILQ SFSRFTRVPS GGYSSINNVQ DPQKPEPRDK MESFFLGETL KYLFLLFSDD PNLLSLDAYV FNTEAHPLPI WTPAVDHHHH HH
• Sequence Length: 699

• Applicable Applications for MAN1B1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for MAN1B1 recombinant protein

Recombinant Human MAN1B1 Protein is produced by our Mammalian expression system and the target gene encoding Asp106-Ala699 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 218749883
• NCBI GeneID: 11253
• NCBI Accession #: NP_057303.2
• NCBI GenBank Nucleotide #: NM_016219.4
• UniProt Accession #: Q9UKM7; Q5VSG3; Q9BRS9; Q9Y5K7
• Molecular Weight: 79,580 Da
• NCBI Official Full Name: endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• NCBI Official Synonym Full Names: mannosidase alpha class 1B member 1
• NCBI Official Symbol: MAN1B1
• NCBI Official Synonym Symbols: MRT15; ERMAN1; ERManI; MANA-ER
• NCBI Protein Information: endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• UniProt Protein Name: Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• Protein Family: Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
• UniProt Gene Name: MAN1B1
• UniProt Synonym Gene Names: ERMan1
• UniProt Entry Name: MA1B1_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011]

Uniprot Description

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2.