Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using MAN1B1 antibody.)
Rabbit anti-Human, Mouse MAN1B1 Polyclonal Antibody | anti-MAN1B1 antibody
MAN1B1 Antibody
Gene Names
MAN1B1; MRT15; ERMAN1; MANA-ER
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
MAN1B1; Polyclonal Antibody; MAN1B1 Antibody; Endoplasmic reticulum mannosyl-oligosaccharide 1; 2-alpha-mannosidase; EC=3.2.1.113; ER alpha-1; 2-mannosidase; anti-MAN1B1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total MAN1B1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
699
Applicable Applications for anti-MAN1B1 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human MAN1B1.
Target Name
MAN1B1
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using MAN1B1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using MAN1B1 antibody.)
Related Product Information for anti-MAN1B1 antibody
Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2.Tremblay L.O., Glycobiology 9:1073-1078(1999) [PubMed: 10521544].Gonzalez D.S., J. Biol. Chem. 274:21375-21386(1999) [PubMed: 10409699].Clark H.F., Genome Res. 13:2265-2270(2003) [PubMed: 12975309].
Product Categories/Family for anti-MAN1B1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,580 Da
NCBI Official Full Name
endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
NCBI Official Synonym Full Names
mannosidase, alpha, class 1B, member 1
NCBI Official Symbol
MAN1B1
NCBI Official Synonym Symbols
MRT15; ERMAN1; MANA-ER
NCBI Protein Information
endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
UniProt Protein Name
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
UniProt Gene Name
MAN1B1
UniProt Synonym Gene Names
ERMan1
UniProt Entry Name
MA1B1_HUMAN
NCBI Description
This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011]
Uniprot Description
Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2.
Research Articles on MAN1B1
Similar Products
Product Notes
The MAN1B1 man1b1 (Catalog #AAA9406686) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAN1B1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAN1B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the MAN1B1 man1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAN1B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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