Mouse RSPO3 ELISA Kit | RSPO3 elisa kit

Mouse RSPO3 ELISA Kit

Cat. Num. AAA8248457

• Gene Names: Rspo3; Thsd2; AW742308; Cristin1; 2810459H04Rik
• Reactivity: Mouse
• Synonyms: RSPO3; Mouse RSPO3 ELISA Kit; PWTSR; THSD2; R-spondin-3; Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2; RSPO3 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse IL-5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse IL-5 proteins within the range of 15.6 pg/ml-1000 pg/ml.
• Sequence Length: 277

• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates
• Sensitivity: 8 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RSPO3 elisa kit

Principle of the Assay: The MyBioSource's Mouse IL-5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-5 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse IL-5 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

NCBI and Uniprot Product Information

• NCBI GI #: 269973856
• NCBI GeneID: 72780
• NCBI Accession #: NP_082627.3
• NCBI GenBank Nucleotide #: NM_028351.3
• UniProt Accession #: Q2TJ95; Q3SYI9; Q5R2V4; Q8BVW2; Q9CSB2; A6H6M4
• Molecular Weight: 31,454 Da
• NCBI Official Full Name: R-spondin-3
• NCBI Official Synonym Full Names: R-spondin 3
• NCBI Official Symbol: Rspo3
• NCBI Official Synonym Symbols: Thsd2; AW742308; Cristin1; 2810459H04Rik
• NCBI Protein Information: R-spondin-3
• UniProt Protein Name: R-spondin-3
• Protein Family: R-spondin
• UniProt Gene Name: Rspo3
• UniProt Synonym Gene Names: Cristin-1
• UniProt Entry Name: RSPO3_MOUSE

Uniprot Description

R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Cellular Component: extracellular region

Molecular Function: frizzled binding; heparin binding; receptor binding

Biological Process: angiogenesis; blood vessel remodeling; sprouting angiogenesis