Mouse FGF23 ELISA Kit | FGF23 elisa kit

Mouse FGF23 ELISA Kit

Cat. Num. AAA8248437

• Reactivity: Mouse
• Synonyms: FGF23; Mouse FGF23 ELISA Kit; HYPF; Fibroblast growth factor 23; FGF-23; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor; FGF23 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CXCL5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CXCL5 proteins within the range of 15.6 pg/ml-1000 pg/ml.
• Sequence Length: 251

• Samples: Cell Culture Supernatants, Serum, Plasma
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF23 elisa kit

Principle of the Assay: The MyBioSource' Mouse CXCL5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CXCL5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

NCBI and Uniprot Product Information

• NCBI GI #: 12083589
• NCBI GeneID: 64654
• NCBI Accession #: NP_073148.1
• NCBI GenBank Nucleotide #: NM_022657.4
• UniProt Accession #: Q9EPC2
• Molecular Weight: 27,758 Da
• NCBI Official Full Name: fibroblast growth factor 23
• NCBI Official Synonym Full Names: fibroblast growth factor 23
• NCBI Official Symbol: Fgf23
• NCBI Protein Information: fibroblast growth factor 23
• UniProt Protein Name: Fibroblast growth factor 23
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf23
• UniProt Synonym Gene Names: FGF-23
• UniProt Entry Name: FGF23_MOUSE

NCBI Description

This gene encodes a member of the fibroblast growth factor family. The encoded protein regulates phosphate homeostasis and vitamin D metabolism. Mutation of the related gene in humans causes autosomal dominant hypophosphatemic rickets (ADHR). The secreted protein is further cleaved into N- and C-terminal chains, which results in loss of function. [provided by RefSeq, Mar 2013]

Uniprot Description

FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: fibroblast growth factor receptor binding; protein binding; type 1 fibroblast growth factor receptor binding

Biological Process: cellular phosphate ion homeostasis; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; negative regulation of bone mineralization; negative regulation of hormone secretion; negative regulation of osteoblast differentiation; phosphate ion homeostasis; positive regulation of transcription, DNA-dependent; regulation of bone mineralization; vitamin D catabolic process