Human Nectin 4 ELISA Kit | NECTIN4 elisa kit

Human Nectin 4 ELISA Kit

Cat. Num. AAA8248380

• Gene Names: NECTIN4; LNIR; PRR4; EDSS1; PVRL4; nectin-4
• Reactivity: Human
• Synonyms: Nectin 4; Human Nectin 4 ELISA Kit; LNIR; PRR4; PVRL4; Poliovirus receptor-related protein 4; Ig superfamily receptor LNIR; Nectin-4; NECTIN4 elisa kit

• Reactivity: Human
• Specificity: The Human Nectin 4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Nectin 4 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 510

• Samples: Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates, Urine
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NECTIN4 elisa kit

Principle of the Assay: The Human Nectin 4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Nectin 4 in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates, Urine. This assay employs an antibody specific for Human Nectin 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Nectin 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Nectin 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Nectin 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

NCBI and Uniprot Product Information

• NCBI GI #: 222136611
• NCBI GeneID: 81607
• NCBI Accession #: NP_112178.2
• NCBI GenBank Nucleotide #: NM_030916.2
• UniProt Accession #: Q96NY8; Q96K15; B4DQW3
• Molecular Weight: 24,341 Da
• NCBI Official Full Name: nectin-4
• NCBI Official Synonym Full Names: nectin cell adhesion molecule 4
• NCBI Official Symbol: NECTIN4
• NCBI Official Synonym Symbols: LNIR; PRR4; EDSS1; PVRL4; nectin-4
• NCBI Protein Information: nectin-4
• UniProt Protein Name: Nectin-4
• Protein Family: Nectin
• UniProt Gene Name: NECTIN4
• UniProt Entry Name: NECT4_HUMAN

NCBI Description

This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]

Uniprot Description

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.