Human LRIG3 ELISA Kit | LRIG3 elisa kit

Human LRIG3 ELISA Kit

Cat. Num. AAA8248376

• Gene Names: LRIG3; LIG3
• Reactivity: Human
• Synonyms: LRIG3; Human LRIG3 ELISA Kit; LIG3; Leucine-rich repeats and immunoglobulin-like domains protein 3; LIG-3; LRIG3 elisa kit

• Reactivity: Human
• Specificity: The Human LRIG3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human LRIG3 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 1059

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <12 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LRIG3 elisa kit

Principle of the Assay: The Human LRIG3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LRIG3 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human LRIG3 coated on a 96-well plate. Standards and samples are pipetted into the wells and LRIG3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LRIG3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LRIG3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northern blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the pattern of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.

NCBI and Uniprot Product Information

• NCBI GI #: 209862903
• NCBI GeneID: 121227
• NCBI Accession #: NP_001129523.1
• NCBI GenBank Nucleotide #: NM_001136051.2
• UniProt Accession #: Q6UXM1; Q6UXL7; Q8NC72
• Molecular Weight: 117,438 Da
• NCBI Official Full Name: leucine-rich repeats and immunoglobulin-like domains protein 3 isoform 1
• NCBI Official Synonym Full Names: leucine rich repeats and immunoglobulin like domains 3
• NCBI Official Symbol: LRIG3
• NCBI Official Synonym Symbols: LIG3
• NCBI Protein Information: leucine-rich repeats and immunoglobulin-like domains protein 3
• UniProt Protein Name: Leucine-rich repeats and immunoglobulin-like domains protein 3
• Protein Family: Leucine-rich repeats and immunoglobulin-like domains protein
• UniProt Gene Name: LRIG3
• UniProt Synonym Gene Names: LIG3; LIG-3
• UniProt Entry Name: LRIG3_HUMAN

Uniprot Description

LRIG3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: extracellular space