Human LRIG3 ELISA Kit | LRIG3 elisa kit

Human LRIG3 PicoKine ELISA Kit

Cat. Num. AAA178548

• Gene Names: LRIG3; LIG3
• Reactivity: Human
• Synonyms: LRIG3; Human LRIG3 PicoKine ELISA Kit; LIG3; LIG 3; LIG-3; LRIG1-3; Lrig3; Q6UXM1; Leucine-rich repeats and immunoglobulin-like domains protein 3; LRIG3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human LRIG3
• Sequence Length: 1059

• Samples: Cell culture supernates, serum and plasma(heparin).
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human LRIG3 PicoKine ELISA Kit standard curve )

Related Product Information for LRIG3 elisa kit

Principle of the Assay: Human LRIG3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LRIG3 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: D28-T807) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LRIG3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human LRIG3 amount of sample captured in plate.

Background/Introduction: LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northern blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the pattern of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.

NCBI and Uniprot Product Information

• NCBI GI #: 209862903
• NCBI GeneID: 121227
• NCBI Accession #: NP_001129523.1
• NCBI GenBank Nucleotide #: NM_001136051.2
• UniProt Accession #: Q6UXM1; Q6UXL7; Q8NC72
• Molecular Weight: 117,438 Da
• NCBI Official Full Name: leucine-rich repeats and immunoglobulin-like domains protein 3 isoform 1
• NCBI Official Synonym Full Names: leucine rich repeats and immunoglobulin like domains 3
• NCBI Official Symbol: LRIG3
• NCBI Official Synonym Symbols: LIG3
• NCBI Protein Information: leucine-rich repeats and immunoglobulin-like domains protein 3
• UniProt Protein Name: Leucine-rich repeats and immunoglobulin-like domains protein 3
• Protein Family: Leucine-rich repeats and immunoglobulin-like domains protein
• UniProt Gene Name: LRIG3
• UniProt Synonym Gene Names: LIG3; LIG-3
• UniProt Entry Name: LRIG3_HUMAN

Uniprot Description

LRIG3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: extracellular space