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Western Blot (WB) (AMBP Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AMBP antibody detected the AMBP protein (arrow).)

Rabbit anti-Human AMBP Polyclonal Antibody | anti-AMBP antibody

AMBP Antibody (N-term)

Gene Names
AMBP; A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
AMBP; Polyclonal Antibody; AMBP Antibody (N-term); Protein AMBP; Alpha-1-microglobulin; Protein HC; Alpha-1 microglycoprotein; Complex-forming glycoprotein heterogeneous in charge; Inter-alpha-trypsin inhibitor light chain; ITI-LC; Bikunin; EDC1; HI-30; Uronic-acid-rich protein; Trypstatin; HCP; ITIL; anti-AMBP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This AMBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-104 amino acids from the N-terminal region of human AMBP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.33 (varies by lot)
Sequence Positions
77-104
Sequence Length
352
Applicable Applications for anti-AMBP antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(AMBP Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AMBP antibody detected the AMBP protein (arrow).)

Western Blot (WB) (AMBP Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AMBP antibody detected the AMBP protein (arrow).)

Immunohistochemistry (IHC)

(AMBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AMBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (AMBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AMBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-AMBP antibody
This gene encodes a complex glycoprotein secreted in
plasma. The precursor is proteolytically processed into distinct
functioning proteins: alpha-1-microglobulin, which belongs to the
superfamily of lipocalin transport proteins and may play a role in
the regulation of inflammatory processes, and bikunin, which is a
urinary trypsin inhibitor belonging to the superfamily of
Kunitz-type protease inhibitors and plays an important role in many
physiological and pathological processes. This gene is located on
chromosome 9 in a cluster of lipocalin genes.
Product Categories/Family for anti-AMBP antibody
References
Olsson, M.G., et al. Radiat. Res. 174(5):590-600(2010)
Allhorn, M., et al. Blood 99(6):1894-1901(2002)
Amoresano, A., et al. Eur. J. Biochem. 267(7):2105-2112(2000)
Xu, Y., et al. J. Mol. Biol. 276(5):955-966(1998)
Vetr, H., et al. Biol. Chem. Hoppe-Seyler 371(12):1185-1196(1990)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
259
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38999
NCBI Official Full Name
protein AMBP preproprotein
NCBI Official Synonym Full Names
alpha-1-microglobulin/bikunin precursor
NCBI Official Symbol
AMBP
NCBI Official Synonym Symbols
A1M; HCP; ITI; UTI; EDC1; HI30; ITIL; IATIL; ITILC
NCBI Protein Information
protein AMBP
UniProt Protein Name
Protein AMBP
Protein Family
UniProt Gene Name
AMBP
UniProt Synonym Gene Names
HCP; ITIL; Protein HC; ITI-LC
UniProt Entry Name
AMBP_HUMAN

NCBI Description

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes. [provided by RefSeq, Jul 2008]

Uniprot Description

AMBP: Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; protein homodimerization activity; IgA binding; heme binding; calcium oxalate binding; calcium channel inhibitor activity

Biological Process: negative regulation of JNK cascade; receptor-mediated endocytosis; viral reproduction; heme catabolic process; negative regulation of immune response; protein catabolic process; female pregnancy; cell adhesion; protein-chromophore linkage

Research Articles on AMBP

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Product Notes

The AMBP ambp (Catalog #AAA9210145) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 77-104. The AMBP Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AMBP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the AMBP ambp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMBP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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