Rabbit Alpha-enolase Polyclonal Antibody | anti-ENO1 antibody

Anti-Alpha-enolase Antibody

Cat. Num. AAA8245793

• Gene Names: ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: Alpha-enolase; Polyclonal Antibody; Anti-Alpha-enolase Antibody; ENO1L1; MBPB1; MPB1; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein; anti-ENO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of Alpha-enolase protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 341

• Applicable Applications for anti-ENO1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB: 1/500 - 1/2000; IF/ICC: 1/20 - 1/100
• Immunogen: Recombinant full length protein of human Alpha-enolase
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Alpha-enolase expression in PC3 (A), Ramos (B), Hela (C), THP1 (D) whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of Alpha-enolase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Related Product Information for anti-ENO1 antibody

Rabbit polyclonal antibody to Alpha-enolase

NCBI and Uniprot Product Information

• NCBI GI #: 319996655
• NCBI GeneID: 2023
• NCBI Accession #: NP_001188412.1
• NCBI GenBank Nucleotide #: NM_001201483.1
• UniProt Accession #: P06733; P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59
• Molecular Weight: 36,928 Da
• NCBI Official Full Name: c-myc promoter-binding protein-1 isoform MBP-1
• NCBI Official Synonym Full Names: enolase 1
• NCBI Official Symbol: ENO1
• NCBI Official Synonym Symbols: NNE; PPH; MPB1; ENO1L1; HEL-S-17
• NCBI Protein Information: alpha-enolase; c-myc promoter-binding protein-1
• UniProt Protein Name: Alpha-enolase
• Protein Family: Alpha-enolase
• UniProt Gene Name: ENO1
• UniProt Synonym Gene Names: ENO1L1; MBPB1; MPB1; NNE
• UniProt Entry Name: ENOA_HUMAN

NCBI Description

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

Uniprot Description

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: EC 4.2.1.11; Lyase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: cytoplasm; cytosol; extracellular space; extrinsic to plasma membrane; M band; membrane; myelin sheath; nucleus; phosphopyruvate hydratase complex

Molecular Function: DNA binding; GTPase binding; magnesium ion binding; phosphopyruvate hydratase activity; protein binding; protein heterodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: gluconeogenesis; in utero embryonic development; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of binding; response to virus; transcription, DNA-dependent