Human stratifin ELISA Kit | SFN elisa kit

Human 14-3-3 protein sigma, SFN ELISA Kit

Cat. Num. AAA909837

• Gene Names: SFN; YWHAS
• Reactivity: Human
• Synonyms: stratifin; Human 14-3-3 protein sigma; SFN ELISA Kit; Human 14-3-3 protein sigma (SFN) ELISA kit; YWHAS; 14-3-3 sigma; SFN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SFN. No significant cross-reactivity or interference between human SFN and analogues was observed.
• Sequence Length: 248

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.78 ng/ml-50 ng/ml
• Sensitivity: < 0.195 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFN elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5454052
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142.3
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: 27,774 Da
• NCBI Official Full Name: 14-3-3 protein sigma
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1
• UniProt Protein Name: 14-3-3 protein sigma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1
• UniProt Entry Name: 1433S_HUMAN

Uniprot Description

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding

Biological Process: positive regulation of protein export from nucleus; release of cytochrome c from mitochondria; regulation of epidermal cell division; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; regulation of cyclin-dependent protein kinase activity; negative regulation of caspase activity; signal transduction; positive regulation of cell growth