Rabbit FKBP6 Polyclonal Antibody | anti-FKBP6 antibody
Anti-FKBP6 Antibody
Western Blot (WB)
(Western blot analysis of FKBP6 expression in HepG2 (A), mouse heart (B), mouse liver (C), rat testis (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of FKBP6 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of FKBP6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cis-trans peptidyl-prolyl isomerase that may function in immunoregulation and basic cellular processes involving protein folding and trafficking. This gene is located in a chromosomal region that is deleted in Williams-Beuren syndrome. Defects in this gene may cause male infertility. There are multiple pseudogenes for this gene located nearby on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
FKBP6: PPIases accelerate the folding of proteins. FKBP6 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of FKBP6 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 5.2.1.8; Isomerase
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum membrane; synaptonemal complex
Molecular Function: FK506 binding; Hsp90 protein binding; peptidyl-prolyl cis-trans isomerase activity; protein binding
Biological Process: cell differentiation; DNA methylation during gametogenesis; meiosis; protein folding; protein peptidyl-prolyl isomerization; RNA-mediated gene silencing; spermatogenesis
