Typical Testing Data/Standard Curve (for reference only)
General Phosphofructokinase Assay Kit | Pfk assay kit
Phosphofructokinase Microplate Assay Kit
Gene Names
Pfk; BcDNA.GH12192; BcDNA:GH12192; CG4001; DmelCG4001; l(2)06339; pfk; PFK; PFK1
Reactivity
General
Applications
Functional Assay
Synonyms
Phosphofructokinase; Phosphofructokinase Microplate Assay Kit; EC 2.7.1.11; PFK; 6-phosphofructokinase; Phosphofructokinase I; Phosphohexokinase; Pfk assay kit
Reactivity
General
Sequence Length
922
Applicable Applications for Pfk assay kit
Functional Assay
Samples
Urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples
Detection Range
4 umol/L - 400 umol/L
Preparation and Storage
Shipped and store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Pfk assay kit
Background/Introduction: Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui's disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for identification, prediction and therapeutic strategy. The Phosphofructokinase Microplate Assay Kit, PFK converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. NADH can be measured at a colorimetric readout at 340 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,720 Da
NCBI Official Full Name
phosphofructokinase, isoform E
NCBI Official Synonym Full Names
Phosphofructokinase
NCBI Official Symbol
Pfk
NCBI Official Synonym Symbols
BcDNA.GH12192; BcDNA:GH12192; CG4001; DmelCG4001; l(2)06339; pfk; PFK; PFK1
NCBI Protein Information
CG4001 gene product from transcript CG4001-RE
UniProt Protein Name
ATP-dependent 6-phosphofructokinase
UniProt Gene Name
Pfk
UniProt Synonym Gene Names
ATP-PFK; Phosphofructokinase
UniProt Entry Name
PFKA_DROME
Uniprot Description
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
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Product Notes
The General Pfk pfk (Catalog #AAA8243182) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8243182 Assay Kit recognizes General Pfk. AAA Biotech's Phosphofructokinase can be used in a range of immunoassay formats including, but not limited to, Functional Assay. Researchers should empirically determine the suitability of the Pfk pfk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphofructokinase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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