Mouse anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody

CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4) (AP)

Cat. Num. AAA6247608

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by protein A affinity chromatography from tissue culture supernatant.
• Synonyms: CD4; Monoclonal Antibody; CD4 (CD4 Antigen; CD4 Molecule; CD4 Receptor; CD4mut; T Cell Surface Antigen T4/Leu3; T Cell Antigen T4; T Cell Surface Glycoprotein CD4) (AP); anti-CD4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3H757
• Specificity: Recognizes human CD4.
• Purity/Purification: Purified by protein A affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CD4 antibody: Immunohistochemistry (IHC)
• Immunogen: Human PHA blasts.
• Conjugate: AP
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4)

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: Alexa Fluor® 488)

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: Pacific Blue®)

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: AZIDE FREE)

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: RPE - Alexa Fluor® 750)

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: RPE-Alexa 647)

Testing Data

(Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4:ALEXA 405)

Product Categories/Family for anti-CD4 antibody

Antibodies; CD Markers

References

1. Zarkesh-Esfahani, H. et al. (2001) High-dose leptin activates human leukocytes via receptor expression on monocytes. J. Immunol.167:4593 - 4599, 2. Voehringer, D. et al. (2002) Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectin-like receptor G1 (KLRG1). Blood.100: 3698 - 3702, 3. Piatier-Tonneau, D. (1997) CD4 workshop panel report. In Leucocyte Typing VI. White cell differentiation antigens. Edited by Kishimoto, T., Kikutani, H., von dem Borne, A.E.G.Kr., Goyert, A.M., Mason, D.Y., Miyasaka, M., Moretta, L., Okumura, K., Shaw, S., Springer, T.A., Sugamura, K., Zola, H. Garland publishing Inc. New York & London.

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation

Disease: Okt4 Epitope Deficiency