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Western Blot (WB) (Western Blot)

Rabbit Cyclin A Polyclonal Antibody

Cyclin A (BSA & Azide Free) (MaxLight 405)

Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Cyclin A; Polyclonal Antibody; Cyclin A (BSA & Azide Free) (MaxLight 405); anti-Cyclin A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Cyclin A. Species Crossreactivity: human, mouse, rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Applicable Applications for anti-Cyclin A antibody
Immunohistochemistry (IHC) Frozen/Paraffin
Application Notes
IHC-F/P: (Ab-6 is better) (Ab 5-10ug/ml for 30 min at RT) ** [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.]
Applications are based on unconjugated antibody.
Immunogen
A synthetic peptide from the C-terminusl of human cyclin A.
Conjugate
MaxLight405
Positive Control
HeLa cells. Tonsil.
Epitope
C-terminal
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot)

Western Blot (WB) (Western Blot)
Product Categories/Family for anti-Cyclin A antibody
References
1. Dutta A; et al. Proc Natl Acad Sci USA, 1995, 92(12):5386-90. 2. Gong J; et al. Leukemia, 1995, 9(5):893-9. Additional: 1. Gong J; Traganos F; Darzynkiewicz Z. Discrimination of G2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. Exp Cell Research, 1995, 220(1):226-31. 2. Gong J; Traganos F; Darzynkiewicz Z. Growth imbalance and altered expression of cyclins B1, A, E, and D3 in MOLT-4 cells synchronized in the cell cycle by inhibitors of DNA replication. Cell Growth and Differentiation, 1995, 6(11):1485-93. 3. Jeffrey PD; Russo AA; Polyak K; Gibbs E; Hurwitz J; Massague J; Pavletich NP. Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex [see comments]. Nature, 1995, 376(6538):313-20. 4. Bodey B; Williams RT; Carbonaro-Hall DA; Horvath A; Tolo VT; Luck JV Jr; Taylor CR; Hall FL. Immunocytochemical detection of cyclin A and cyclin D in formalin-fixed, paraffin-embedded tissues: novel, pertinent markers of cell proliferation. Modern Pathology, 1994, 7(8):846-52. 5. Inohara S; Kitano Y. Immunohistochemical detection of cyclin D and cyclin A in human hyperproliferative epidermis. Archives for Dermatological Research. Archiv fur Dermatologische Forschung, 1994, 286(8):504-6. 6. Adamczewski JP; Gannon JV; Hunt T. Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 1993, 67(11):6551-7. 7. Faha B; Harlow E; Lees E. The adenovirus E1A-associated kinase consists of cyclin Ep33cdk2 and cyclin A-p33cdk2. J of Virol, 1993, 67:2456-65. 8. Lees EM; Harlow E. Sequences within the conserved cyclin box of human cyclin A are sufficient for binding to and activation of cdc2 kinase. Mol and Cell Biol, 1993, 13(2):1194-201. 9. Tommasino M; Adamczewski JP; Carlotti F; Barth CF; Manetti R; Contorni M; Cavalieri F; Hunt T; Crawford L. HPV16 E7 protein associates with the protein kinase p33CDK2 and cyclin A. Oncogene, 1993, 8(1):195-202. 10. Woodman PG; Adamczewski JP; Hunt T; Warren G. In vitro fusion of endocytic vesicles is inhibited by cyclin A-cdc2 kinase. Molecular Biology of the Cell, 1993, 4(5):541-53. 11. Ewen ME; Faha B; Harlow E; Livingston DM. Interaction of p107 with cyclin A independent of complex formation with viral oncoproteins. Science, 1992, 255(5040):85-7. 12. Faha B; Ewen ME; Tsai LH; Livingston DM; Harlow E. Interaction between human cyclin A and adenovirus E1A-associated p107 protein. Science, 1992, 255(5040):87-90. 13. Kobayashi H; Stewart E; Poon R; Adamczewski JP; Gannon J; Hunt T. Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits. Molecular Biology of the Cell, 1992, 3(11):1279-94. 14. Lees E; Faha B; Dulic V; Reed SI; Harlow E. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner. Genes and Development, 1992 Oct, 6(10):1874-85. 15. Williams RT; Carbonaro-Hall DA; Hall FL. Copurification of p34cdc2/p58cyclin A prolinedirected protein kinase and the retinoblastoma tumor susceptibility gene product: interaction of an oncogenic serine/threonine protein kinase with a tumor-suppressor protein. Oncogene, 1992, 7:423-32.

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Product Notes

The Cyclin A (Catalog #AAA6471135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyclin A (BSA & Azide Free) (MaxLight 405) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Frozen/Paraffin. IHC-F/P: (Ab-6 is better) (Ab 5-10ug/ml for 30 min at RT) ** [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Cyclin A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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