TRPA1 sirna

TRPA1 siRNA (Rat)

Cat. Num. AAA8239122

• Gene Names: Trpa1; Anktm1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TRPA1; TRPA1 siRNA (Rat); ANKTM1; Transient receptor potential cation channel subfamily A member 1; Ankyrin-like with transmembrane domains protein 1; TRPA1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: TRPA1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1125

• Applicable Applications for TRPA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat TRPA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TRPA1 sirna

siRNA to inhibit TRPA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46485385
• NCBI GeneID: 312896
• NCBI Accession #: NP_997491.1
• NCBI GenBank Nucleotide #: NM_207608.1
• UniProt Accession #: Q6RI86
• Molecular Weight: 128,602 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily A member 1
• NCBI Official Synonym Full Names: transient receptor potential cation channel, subfamily A, member 1
• NCBI Official Symbol: Trpa1
• NCBI Official Synonym Symbols: Anktm1
• NCBI Protein Information: transient receptor potential cation channel subfamily A member 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily A member 1
• Protein Family: Transient receptor potential cation channel subfamily
• UniProt Gene Name: Trpa1
• UniProt Synonym Gene Names: Anktm1
• UniProt Entry Name: TRPA1_RAT

Uniprot Description

Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, cinnamaldehyde, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana (PubMed:14712238). Is also activated by menthol (in vitro) (). May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system ().