SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
DNAPK recombinant protein
DNAPK Protein
Applications
Western Blot
Synonyms
DNAPK; DNAPK Protein; PRKDC; HYRC; p350; DNA-PK; DNPK1; HYRC1; XRCC7; DNAPK recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for DNAPK recombinant protein
Western Blot (WB)
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for DNAPK recombinant protein
Recombinant human DNAPK (3746-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. This gene accession number is NM_006904.
DNAPK is a member of the PI3/PI4-kinase family which encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK), which is involved in DNA nonhomologous end-joining (NHEJ) during DNA double-strand break (DSB) repair and for V(D)J recombination during immune development. The second component of DNA-PK is Ku, which is required for proper activation of PRKDC (1). The initial events in retroviral integration are detected as DNA damage by the host cell, and that completion of the integration process requires the DNA-PK-mediated repair pathway (2).
DNAPK is a member of the PI3/PI4-kinase family which encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK), which is involved in DNA nonhomologous end-joining (NHEJ) during DNA double-strand break (DSB) repair and for V(D)J recombination during immune development. The second component of DNA-PK is Ku, which is required for proper activation of PRKDC (1). The initial events in retroviral integration are detected as DNA damage by the host cell, and that completion of the integration process requires the DNA-PK-mediated repair pathway (2).
Product Categories/Family for DNAPK recombinant protein
References
1. Van der Burg, M.et.al: A DNA-PKcs mutation in a radiosensitive T-B-SCID patient inhibits Artemis activation and nonhomologous end-joining. J. Clin. Invest. 119: 91-98, 2009.
2. Daniel, R.et.al: A role for DNA-PK in retroviral DNA integration. Science 284: 644-647, 1999.
2. Daniel, R.et.al: A role for DNA-PK in retroviral DNA integration. Science 284: 644-647, 1999.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DNAPK (Catalog #AAA516779) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNAPK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DNAPK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAPK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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