DRD5 sirna

DRD5 siRNA (Mouse)

Cat. Num. AAA8238926

• Gene Names: Drd5; D5R; Drd-5; Drd1b; Gpcr1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DRD5; DRD5 siRNA (Mouse); D(1B) dopamine receptor; D(5) dopamine receptor; Dopamine D5 receptor; DRD5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DRD5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 478

• Applicable Applications for DRD5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DRD5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DRD5 sirna

siRNA to inhibit DRD5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 29789042
• NCBI GeneID: 13492
• NCBI Accession #: NP_038531.1
• NCBI GenBank Nucleotide #: NM_013503.3
• UniProt Accession #: Q8BLD9; Q61439; Q80UB7
• Molecular Weight: 53,076 Da
• NCBI Official Full Name: D(1B) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D5
• NCBI Official Symbol: Drd5
• NCBI Official Synonym Symbols: D5R; Drd-5; Drd1b; Gpcr1
• NCBI Protein Information: D(1B) dopamine receptor
• UniProt Protein Name: D(1B) dopamine receptor
• Protein Family: D(1B) dopamine receptor
• UniProt Gene Name: Drd5
• UniProt Entry Name: DRD5_MOUSE

Uniprot Description

DRD5: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Defects in DRD5 are a cause of benign essential blepharospasm (BEB). BEB is a primary focal dystonia affecting the orbicularis oculi muscles. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. BEB usually begins in middle age. Initial symptoms include eye irritation and frequent blinking, progressing to involuntary spasms of eyelid closure. Patients have normal eyes. The visual disturbance is due solely to the forced closure of the eyelids. In severe cases, this can lead to functional blindness. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Cellular Component: membrane; cell soma; brush border membrane; axon; integral to plasma membrane; dendrite; integral to membrane; plasma membrane; dendritic spine

Molecular Function: G-protein coupled receptor activity; signal transducer activity; dopamine receptor activity; dopamine binding; G-protein alpha-subunit binding; dopamine D1 receptor-like receptor activity; drug binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic transmission, dopaminergic; wound healing; norepinephrine-epinephrine vasoconstriction involved in regulation of systemic arterial blood pressure; signal transduction; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; positive regulation of cAMP biosynthetic process; visual learning; positive regulation of adenylate cyclase activity; negative regulation of blood pressure; negative regulation of NAD(P)H oxidase activity; negative regulation of cell migration; associative learning; dopamine receptor, adenylate cyclase activating pathway; response to amphetamine; mating behavior; transmission of nerve impulse; response to cocaine; G-protein coupled receptor protein signaling pathway; regulation of female receptivity; regulation of systemic arterial blood pressure by vasopressin; sensitization; dopamine receptor signaling pathway; negative regulation of protein kinase activity